DBF2, a cell cycle-regulated protein kinase, is physically and functionally associated with the CCR4 transcriptional regulatory complex
DBF2, a cell cycle-regulated protein kinase, is physically and functionally associated with the CCR4 transcriptional regulatory complex
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DOI：
10.1093/emboj/16.17.5289
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发表时间：
1997-09-01
期刊：
影响因子：
11.4
通讯作者：
Denis, CL
中科院分区：
文献类型：
--
作者：
Liu, HY;Toyn, JH;Denis, CL
CCR4是一种影响酵母中多种基因表达的通用转录调控因子，在体内形成一个多亚基复合体。通过酵母双杂交筛选，我们鉴定了细胞周期调节蛋白激酶DBF2是CCR4相关蛋白，DBF2是细胞周期向G(1)期转变所必需的，DBF2与CCR4和CCR4相关因子CAF1/POP2免疫共沉淀，并与CCR4复合体共纯化，此外，DBF2的干扰导致了与菌株相似的表型和转录缺陷；另一方面，缺乏CCR4或CAF1的CCR4和CAF1突变被发现以与观察到的DBF2缺陷相似的方式影响细胞周期进展，这些数据表明DBF2参与了基因表达的控制，并提示CCR4复合体在细胞周期的有丝分裂晚期调节转录。
CCR4, a general transcriptional regulator affecting the expression of a number of genes in yeast, forms a multi-subunit complex in vivo, Using the yeast two-hybrid screen, we have identified DBF2, a cell cycle-regulated protein kinase, as a CCR4-associated protein, DBF2 is required for cell cycle progression at the telophase to G(1) cell cycle transition, DBF2 co-immunoprecipitated with CCR4 and CAF1/POP2, a CCR4-associated factor, and co-purified with the CCR4 complex, Moreover, a dbf2 disruption resulted in phenotypes and transcriptional defects similar to those observed in strains; deficient for CCR4 or CAF1, ccr4 and caf1 mutations, on the other hand, were found to affect cell cycle progression in a manner similar to that observed for dbf2 defects, These data indicate that DBF2 is involved in the control of gene expression and suggest that the CCR4 complex regulates transcription during the late mitotic part of the cell cycle.