The matrix interference to the immunoassay of food samples: The effect of some proteins in aquatic products
The matrix interference to the immunoassay of food samples: The effect of some proteins in aquatic products
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DOI：
10.1080/09540105.2015.1086317
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发表时间：
2016-03-03
影响因子：
3
通讯作者：
Sui, Jianxin
中科院分区：
文献类型：
--
作者：
Wang, Xiudan;Lin, Hong;Sui, Jianxin
消除基质干扰是免疫分析进一步发展和应用的任务。采用免疫印迹和竞争性间接酶标免疫吸附试验研究了水样中蛋白质对免疫分析的影响。比目鱼中的一些蛋白质与不同的抗体相互作用，导致免疫分析的准确性大大降低。此外，这些基质蛋白更喜欢与酶标记的抗体相互作用，而不是无标记的抗体。用不同的鱼和虾样品验证了这种基质蛋白-免疫球蛋白的相互作用，并观察到了相似的结果，这表明蛋白质诱导的基质干扰对免疫分析具有普遍意义。此外，还对防止这种干扰的策略进行了初步探讨。
The elimination of matrix interference is now a task for further development and application of immunoassays. Here the effect of proteins in aquatic samples on immunoassay was investigated by western blotting and competitive indirect enzyme-labeled immunosorbent assay (ci-ELISA). Some proteins in flatfishes interacted with different antibodies, resulting in significant loss of accuracy of the immunoassays. Moreover, these matrix proteins prefer to interact with enzyme-labeled antibodies than label-free ones. Such a matrix protein-immunoglobulin interaction was validated with different fish and shrimp samples and similar results were observed, which demonstrated a universal significance of the protein-induced matrix interference to immunoassays. Besides, the strategies to prevent such interference were preliminarily discussed.