Comparison of Southern transfer hybridization and dot filter hybridization for detection of cervical human papillomavirus infection with types 6, 11, 16, 18, 31, 33, and 35.
Comparison of Southern transfer hybridization and dot filter hybridization for detection of cervical human papillomavirus infection with types 6, 11, 16, 18, 31, 33, and 35.
复制标题
Southern转移杂交与斑点滤光杂交检测宫颈人乳头瘤病毒6、11、16、18、31、33、35型感染的比较。
DOI：
10.1093/ajcp/94.5.561
复制
发表时间：
1990
影响因子：
3.5
通讯作者：
Holmes,KK
中科院分区：
文献类型：
--
作者：
Kiviat,NB;Koutsky,LA;Critchlow,CW;Galloway,DA;Vernon,DA;Peterson,ML;McElhose,PE;Pendras,SJ;Stevens,CE;Holmes,KK
比较了市售斑点过滤杂交试剂盒（Virapap®试剂盒）与Southern转移杂交法检测450例连续就诊于性传播疾病诊所的女性宫颈标本中7种类型的人乳头瘤病毒（HPV）。与Southern转移杂交相比，用斑点过滤试剂盒中使用的相同探针进行，斑点过滤杂交的敏感性、特异性、阳性预测值和阴性预测值分别为90%、94%、74%和98%。在宫颈细胞学异常的患者中，HPVDNA的检出率为44%，Southern转移杂交为35%。尽管26%的斑点杂交阳性标本未被Southern转移杂交证实，但仅用斑点杂交检测HPV DNA的20例中有5例（25%）检出宫颈不典型增生，相比之下，25（8%）的人没有明确的HPV证据，两种方法均检出HPV DNA的53例中有16例（30%）（P = 0.009）。两种方法的观察者间和观察者内印迹判读重现性的kappa统计量相似。斑点过滤杂交法评价似乎是一个令人满意的替代Southern转移杂交检测HPV DNA。
A commercial dot filter hybridization kit (Virapap® Kit) was compared with Southern transfer hybridization for the detection of seven types of human papillomavirus (HPV) in cervical specimens from 450 consecutive females attending a sexually transmitted diseases clinic. In comparison with Southern transfer hybridization, performed with the same probes used in the dot filter kit, the sensitivity, specificity, and positive and negative predictive values of dot filter hybridization were 90%, 94%, 74%, and 98%, respectively. Among patients with cervical cytologic dysplasia, HPV DNA was detected in 44% by dot filter hybridization and in 35% by Southern transfer hybridization. Although 26% of specimens positive by dot filter hybridization were not confirmed by Southern transfer hybridization, cervical dysplasia was detected in 5 (25%) of 20 with HPV DNA detected by dot filter hybridization alone, compared with 25 (8%) of those with no definitive evidence of HPV by either method (P = 0.009) and with 16 (30%) of 53 with HPV DNA detected by both methods (P = 0.7). The kappa statistic for interobserver and intraobserver reproducibility for interpretation of blots was similar for the two methods. The dot filter hybridization method evaluated appears to be a satisfactory alternative to Southern transfer hybridization for detection of HPV DNA.