Transcriptomics Identify CD9 as a Marker of Murine IL-10-Competent Regulatory B Cells.
Transcriptomics Identify CD9 as a Marker of Murine IL-10-Competent Regulatory B Cells.
复制标题
DOI：
10.1016/j.celrep.2015.09.070
复制
发表时间：
2015-11-10
期刊：
影响因子：
8.8
通讯作者：
Basu U
中科院分区：
文献类型：
--
作者：
Sun J;Wang J;Pefanis E;Chao J;Rothschild G;Tachibana I;Chen JK;Ivanov II;Rabadan R;Takeda Y;Basu U
调节性B细胞（布雷格）在各种自身免疫/炎症模型和疾病中具有免疫抑制功能，并且被发现富集在不同的B细胞亚群中。缺乏有效鉴定布雷格细胞的独特标志物或标志物组阻碍了对其起源、发育和免疫学作用的详细研究。在此，我们对表达IL 10的B细胞进行转录组分析，以确定布雷格生物发生和功能的关键调节因子，并确定CD 9（一种四跨膜蛋白家族跨膜蛋白）作为大多数小鼠IL 10 + B细胞及其祖细胞的关键表面标志物。在离体T细胞增殖测定中，CD 9通过依赖于B/T细胞相互作用的机制在IL 10 + B细胞的抑制功能中起作用。在体内模型系统中，CD 9 + B细胞也表现出对Th 1介导的接触性超敏反应的抑制。综上所述，我们的研究结果表明，CD 9参与了调节性B细胞的免疫抑制活性。
Regulatory B cells (Breg) have immune suppressive functions in various autoimmune/inflammation models and diseases, and are found enriched in diverse B-cell subsets. The lack of a unique marker or set of markers efficiently identifying Breg cells impedes detailed investigation into their origin, development, and immunological roles. Here, we perform transcriptome analysis of IL10-expressing B cells to identify key regulators for Breg biogenesis and function and identify CD9, a tetraspanin-family transmembrane protein, as a key surface marker for most mouse IL10+ B cells and their progenitors. CD9 plays a role in the suppressive function of IL10+ B cells in ex-vivo T cell proliferation assays through a mechanism that is dependent upon B/T cell interactions. CD9+ B cells also demonstrate inhibition of Th1 mediated contact hypersensitivity in an in vivo model system. Taken together, our findings implicate CD9 in the immunosuppressive activity of regulatory B cells.