Construction of a highly sensitive non-enzymatic sensor for superoxide anion radical detection from living cells
Construction of a highly sensitive non-enzymatic sensor for superoxide anion radical detection from living cells
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构建用于活细胞超氧阴离子自由基检测的高灵敏度非酶传感器
DOI：
10.1016/j.bios.2016.11.015
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发表时间：
2017-04-15
影响因子：
12.6
通讯作者：
Lu, Xiaoquan
中科院分区：
文献类型：
--
作者：
Liu, Yuelin;Liu, Xiuhui;Lu, Xiaoquan
基于Ag纳米颗粒（NPs）/L-半胱氨酸功能化碳纳米管（Cys-MWCNTs）纳米复合材料，制备了一种新型的非酶超氧阴离子（O-2（中心点-））传感器，并用于测量活细胞中O-2（中心点-）的释放。在这种策略中，AgNPs可以均匀地电沉积在MWCNTs表面，平均直径约为20 nm，通过扫描电子显微镜（SEM）显示。电化学研究表明，AgNPs/Cys-MWCNTs修饰玻碳电极对O-2（中心点）的还原具有良好的催化活性，其线性范围为7.00 × 10（-11）~ 7.41 × 10（-5）M，检出限为2.33 × 10（-11）M（S/N=3）。同时，还首次提出了O-2（中心点）还原的机理.重要的是，这种新型的非酶O-2（中心点）-传感器可以检测O-2（中心点）-释放从癌细胞在外部刺激和正常条件下，这在临床诊断评估活细胞的氧化应激具有很大的潜在应用。
A novel non-enzymatic superoxide anion (O-2(center dot-)) sensor was fabricated based on Ag nanoparticles (NPs)/L-cysteine functioned carbon nanotubes (Cys-MWCNTs) nanocomposites and used to measure the release of O-2(center dot-) from living cells. In this strategy, AgNPs could be uniformly electrodeposited on the MWCNTs surface with average diameter of about 20 nm as exhibited by scanning electronmicroscopy (SEM). Electrochemical study demonstrated that the AgNPs/Cys-MWCNTs modified glassy carbon electrode exhibited excellent catalytic activity toward the reduction of O-2(center dot)- with a super wide linear range from 7.00 x 10(-11) to 7.41x10(-5) M and a low detection limit (LOD) of 2.33x10(-11)M (S/N=3). Meanwhile, the mechanism for O-2(center dot)- reduction was also proposed for the first time. Importantly, this novel non-enzymatic O-2(center dot)- sensor can detect O-2(center dot)- release from cancer cells under both the external stimulation and the normal condition, which has the great potential application in clinical diagnostics to assess oxidative stress of living cells.