Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.
Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.
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DOI：
10.1016/j.carbpol.2016.11.088
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发表时间：
2017-02
影响因子：
11.2
通讯作者：
Hui Zhang;Shaoping Nie;S. Cui;Ming Xu;Huihuang H. Ding;M. Xie
中科院分区：
文献类型：
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作者：
Hui Zhang;Shaoping Nie;S. Cui;Ming Xu;Huihuang H. Ding;M. Xie
利用高效阴离子交换层析(HPAEC)、甲基化和GLC-MS分析以及一维和二维核磁共振波谱，系统地重新鉴定了黑灵芝活性多糖(PSG-1)的主链和支链结构。单糖组成分析表明，PSG-1-F0.2组分主要由葡萄糖(73.8%)和葡萄糖醛酸(15.3%)组成，并含有少量甘露糖(5.7%)和半乳糖(5.2%)。基于甲基化、多步部分酸解和核磁共振研究，提出了β-(1-→-3)-葡聚糖在Theo-6位的取代方法。少量的甘露糖和半乳糖残留物被认为来自PSG中的其他组分，很难从PSG-1-F0.2中分离出来。这种酸性的β-(1-→-3，1-→-6)-葡聚糖结构被认为比PSG-1的先前提议更准确。
The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide fromGanoderma atrum(PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC–MS analysis, and 1D & 2D NMR spectroscopy. Monosaccharide composition analysis revealed that PSG-1-F0.2fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%). Based on methylation, multistep partial acid hydrolysis and NMR study, were proposed to substitute at theO-6 position of β-(1 → 3)-glucan. The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2. This revised structure as an acidic β-(1 → 3, 1 → 6)-glucan is considered to be more accurate than the previous proposal of PSG-1.