The association of interleukin-16 polymorphisms with IL-16 serum levels and risk of colorectal and gastric cancer
The association of interleukin-16 polymorphisms with IL-16 serum levels and risk of colorectal and gastric cancer
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DOI：
10.1093/carcin/bgn281
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发表时间：
2009-02-01
期刊：
影响因子：
4.7
通讯作者：
Zhang, Lin
中科院分区：
文献类型：
--
作者：
Gao, Lin-Bo;Rao, Li;Zhang, Lin
白细胞介素（IL）-16是一种多功能细胞因子，在炎症性疾病以及肿瘤的发生和发展中起着重要作用。IL - 16基因DNA序列的遗传变异可能导致细胞因子产生和/或活性改变，这种变异可能调节个体对结直肠癌（CRC）和胃癌（GC）的易感性。为了验证这一假设，我们在中国人群中研究了IL - 16基因多态性与血清IL - 16水平以及CRC和GC风险之间的关联。我们使用聚合酶链反应 - 限制性片段长度多态性和DNA测序方法分析了596例癌症患者（376例CRC患者和220例GC患者）以及480例年龄和性别匹配的对照者的IL - 16基因单核苷酸多态性。采用酶联免疫吸附测定法测量血清IL - 16水平。IL - 16基因的rs11556218 T/G多态性与CRC和GC患者的易感性显著相关。携带G等位基因的男性和女性患者患CRC和GC的风险明显高于携带T等位基因的个体。另外，携带T等位基因（rs4072111 C/T）的女性与携带C等位基因的个体相比，CRC和GC的患病风险降低。在CRC或GC患者中，IL - 16血清水平明显高于健康对照者，尽管未观察到IL - 16多态性与血清IL - 16水平之间存在显著关联。我们的数据表明IL - 16多态性可能导致CRC和GC易感性增加。
Interleukin (IL)-16, a multifunctional cytokine, plays a fundamental role in inflammatory diseases, as well as in the development and progression of tumors. Genetic variation in the DNA sequence of the IL-16 gene may lead to altered cytokine production and/or activity, and this variation may modulate an individual's susceptibility to both colorectal cancer (CRC) and gastric cancer (GC). To test this hypothesis, we investigated the association of IL-16 gene polymorphisms with serum levels of IL-16 and the risk of CRC and GC in a Chinese population. We analyzed single-nucleotide polymorphisms of the IL-16 gene in 596 cancer patients (376 patients with CRC and 220 patients with GC), and also in 480 age- and sex-matched controls using polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing methods. Serum IL-16 levels were measured by enzyme-linked immunosorbent assay. The rs11556218 T/G polymorphism of the IL-16 gene was significantly associated with the susceptibility to CRC and GC patients. Both male and female patients carrying the G allele had a significantly higher risk for developing CRC and GC compared with individuals carrying the T allele. Alternatively, women carrying the T allele (rs4072111 C/T) showed a decreased risk for CRC and GC compared with individuals carrying the C allele. In patients with CRC or GC, IL-16 serum levels were significantly higher than those in the healthy controls, although no significant association between IL-16 polymorphisms and serum levels of IL-16 was observed. Our data indicate that IL-16 polymorphisms may contribute to CRC and GC susceptibility.