NONISOTOPIC ULTRASTRUCTURAL MAPPING OF TRANSCRIPTION SITES WITHIN THE NUCLEOLUS
NONISOTOPIC ULTRASTRUCTURAL MAPPING OF TRANSCRIPTION SITES WITHIN THE NUCLEOLUS
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DOI：
10.1006/excr.1993.1247
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发表时间：
1993-09-01
影响因子：
3.7
通讯作者：
RASKA, I
中科院分区：
文献类型：
--
作者：
DUNDR, M;RASKA, I
本文介绍了一种非放射性超微结构方法，该方法基于将5-溴尿苷-5 ′-三磷酸掺入到经链球菌溶血素O透化的培养HeLa细胞的RNA中，并用于rRNA转录位点的可视化。即使该方法提供了更好的分辨率比超微结构放射自显影，所获得的结果不允许分配的rRNA转录到一个单一的核仁结构组件。我们将rRNA转录位点定位在具有致密纤维成分的纤维中心的边界区域。此外，该方法还为原位免疫检测核仁RNA合成提供了一种方便的工具。
A nonradioactive ultrastructural method based on the incorporation of 5-bromouridine-5′-triphosphate into the RNA of streptolysin O-permeabilized cultured HeLa cells is described and used for the visualization of rRNA transcription sites. Even though the method provides much better resolution than ultrastructural autoradiography, the results obtained do not allow the assignment of rRNA transcription to a single nucleolar structural component. We locate the rRNA transcription sites at the border region of fibrillar centers with dense fibrillar components. In addition, the method represents a convenient tool for thein situimmunodetection of extranucleolar RNA synthesis.