Genomic analysis of host-pathogen interaction between Fusarium graminearum and wheat during early stages of disease development
Genomic analysis of host-pathogen interaction between Fusarium graminearum and wheat during early stages of disease development
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DOI：
10.1099/mic.0.28750-0
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发表时间：
2006-06-01
期刊：
影响因子：
2.8
通讯作者：
Kistler, H. Corby
中科院分区：
文献类型：
--
作者：
Goswami, Rubella S.;Xu, Jin-Rong;Kistler, H. Corby
禾谷镰刀菌菌株负责引起植物疾病镰刀菌头枯病在它们引起疾病和产生真菌毒素的能力上变化很大。为了了解与致病性相关的真菌基因表达，通过抑制性消减杂交使用接种有高度侵袭性菌株的小麦头和水或该病原体的侵袭性较低的菌株来创建三个cDNA文库。84个真菌基因在最初的疾病发展过程中表达进行了鉴定。其中49个基因的生物信息学功能可以推测。35个EST在现有数据库中没有已知的同源物，也没有通过从头算基因预测方法进行鉴定。这些EST可能代表F.这些基因以前没有被注释过。选择其中一个文库中的四个基因进行靶向基因置换，从而表征参与真菌致病性的双组分反应调节剂同源物。该基因的突变体表现出减少孢子形成和小麦赤霉病的延迟传播。
Fusarium graminearum strains responsible for causing the plant disease Fusarium head blight vary greatly in their ability to cause disease and produce mycotoxins on wheat. With the goal of understanding fungal gene expression related to pathogenicity, three cDNA libraries were created by suppression subtractive hybridization using wheat heads inoculated with a highly aggressive strain and either water or a less aggressive strain of this pathogen. Eighty-four fungal genes expressed during initial disease development were identified. The probable functions of 49 of these genes could be inferred by bioinformatic analysis. Thirty-five ESTs had no known homologues in current databases and were not identified by ab initio gene prediction methods. These ESTs from infected wheat heads probably represent F. graminearum genes that previously were not annotated. Four genes represented in one of these libraries were selected for targeted gene replacement, leading to the characterization of a two-component response regulator homologue involved in pathogenicity of the fungus. The mutants for this gene showed reduced sporulation and delayed spread of Fusarium head blight on wheat.