Toxicological effects of ciprofloxacin exposure to Drosophila melanogaster
Toxicological effects of ciprofloxacin exposure to Drosophila melanogaster
复制标题
环丙沙星暴露对黑腹果蝇的毒理学影响
DOI：
10.1016/j.chemosphere.2019.124542
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发表时间：
2019-12-01
期刊：
影响因子：
8.8
通讯作者：
Wang, Xing
中科院分区：
文献类型：
--
作者：
Liu, Jinyue;Li, Xiaoqin;Wang, Xing
环丙沙星（CIP）的滥用可能会导致严重的副作用，这些作用的机制尚不清楚。本文测定了CIP对果蝇48 h、72 h和96 h的LC_（50）值，并研究了CIP对果蝇的毒副作用。将黑腹果蝇暴露于亚致死浓度（3.2 mg mL（-1））的CIP。处理过的个体寿命较短，发育延迟，许多处理过的幼虫未能化蛹或孵化。结果表明，CIP处理后各虫态的个体变小，蛹的大小和3龄幼虫的体重均表现出较好的剂量-反应关系，即CIP浓度越高，个体变小或体重越轻。此外，降低脂肪体细胞活力，氧化应激标志物（SOD和CAT）和下调的diapl，前，约克（Yki）的两个靶基因，观察到CIP暴露。最重要的是，我们在果蝇中发现了两种类型的黑点，并且具有黑点的幼虫的比例与处理剂量呈正相关，这在该领域是新的。本研究为喹诺酮类药物滥用的潜在危害提供了科学依据，旨在促使临床谨慎使用抗生素。(C)2019爱思唯尔有限公司版权所有。
The abuse of ciprofloxacin (CIP) may cause serious side effects and the mechanisms underlying these effects remain unclear. Here, we determinate the 48 h, 72 h and 96 h LC50 values of CIP to Drosophila melanogaster and demonstrate a series of adverse effects after D. melanogaster was exposed to CIP at a sublethal concentration (3.2 mg mL(-1)). Treated individuals showed shorter lifespan, delayed development and many of the treated larvae failed to pupate or hatch. Smaller body size was observed at every life stage when exposed to CIP and the size of pupae, the weight of third-instar larvae exhibited a perfectly dose-response relationship that the larger concentration exposed to, the smaller body size or lighter weight is. Moreover, reduction in fat body cell viability, elevated oxidative stress markers (SOD and CAT) and down-regulation of diapl, ex, two target genes of Yorkie (Yki), was observed in response to CIP exposure. Most importantly, we found two types of black spot in Drosophila and the proportion of larvae with a black spot was positively related to the treatment dose, which is new in the field. This study provides a scientific basis for the potential harm caused by abuse of quinolones with the goal of urging cautious use of antibiotics. (C) 2019 Elsevier Ltd. All rights reserved.