Synthesis of biodiesel in column fixed-bed bioreactor using the fermented solid produced by Burkholderia cepacia LTEB11
Synthesis of biodiesel in column fixed-bed bioreactor using the fermented solid produced by Burkholderia cepacia LTEB11
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DOI：
10.1016/j.procbio.2010.05.004
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发表时间：
2010-08-01
影响因子：
4.4
通讯作者：
Krieger, Nadia
中科院分区：
文献类型：
--
作者：
Chan Salum, Thais Fabiana;Villeneuve, Pierre;Krieger, Nadia
利用洋葱伯克霍尔德氏菌固态发酵生产脂肪酶，并在固定床反应器中催化合成生物柴油。在固态发酵阶段，蔗渣与葵花籽粕的质量比为50：50，发酵96h，每克干固体可获得234个单位的pNPP水解酶活性，发酵后的固体经冷冻干燥、脱脂后装入柱中，在无共溶剂的介质中催化大豆油乙醇解合成生物柴油，反应混合物在柱中连续循环。反应温度为50℃，醇油摩尔比为3：1，两步加醇，加水1%(m/m)，反应46h，转化率可达95%。该系统有可能降低酶催化的酯交换反应的成本。(C)2010爱思唯尔有限公司。保留所有权利。
We produced a lipase from Burkholderia cepacia in solid-state fermentation and used it to catalyze the synthesis of biodiesel in a fixed-bed reactor. In the solid-state fermentation step, a 50:50 (by mass) mixture of sugarcane bagasse and sunflower seed meal gave 234 units of pNPP-hydrolyzing activity per gram of dry solids at 96 h. This fermented solid was lyophilized and delipidated, packed into a column and used to catalyze the synthesis of biodiesel through the ethanolysis of soybean oil in a medium free of co-solvents, with the reaction mixture being continuously circulated through the column. The best conversion was 95% after 46 h, which was obtained at 50 C, with an alcohol:oil molar ratio of 3:1, alcohol addition in two steps and the addition of 1% of (m/m) water to the reaction medium. This system has potential to decrease the costs of enzyme-catalyzed transesterification reactions. (C) 2010 Elsevier Ltd. All rights reserved.