Anticancer effect of tectochrysin in colon cancer cell via suppression of NF-kappaB activity and enhancement of death receptor expression.
Anticancer effect of tectochrysin in colon cancer cell via suppression of NF-kappaB activity and enhancement of death receptor expression.
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DOI：
10.1186/s12943-015-0377-2
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发表时间：
2015-06-30
期刊：
影响因子：
37.3
通讯作者：
Hong JT
中科院分区：
文献类型：
--
作者：
Park MH;Hong JE;Park ES;Yoon HS;Seo DW;Hyun BK;Han SB;Ham YW;Hwang BY;Hong JT
黄酮类化合物是一种天然酚类化合物，通常存在于水果和蔬菜中。流行病学研究表明，黄酮类化合物还可以降低患结肠癌的风险。摘要木黄酮是益智中的主要黄酮类化合物之一。然而，tectochryin在结肠癌细胞中的抗癌作用及其分子机制尚未有报道。我们研究了在1、5、10 μg/ml的tectochryin是否可以抑制结肠癌细胞的生长。在体内研究中，我们向每只小鼠注射5 mg/kg的tectochryin治疗剂量。tectochryin抑制SW 480和HCT 116人结肠癌细胞的生长。DR 3、DR 4和Fas表达明显增加，促凋亡蛋白表达也明显增加。冬青素处理还抑制NF-κB的活性。对接模型表明，tectochryin直接结合到p50单位。在体内，当用tectochryin处理时，小鼠中的肿瘤重量和体积减小。tectochryin通过抑制NF-κB激活死亡受体表达导致结肠癌细胞凋亡。冬青素可以是用于治疗结肠癌细胞生长的有用试剂以及用于化学抗性癌细胞生长的佐剂。
Flavonoids are a diverse family of natural phenolic compounds commonly found in fruits and vegetables. Epidemiologic studies showed that flavonoids also reduce the risk of colon cancer. Tectochrysin is one of the major flavonoids of Alpinia oxyphylla Miquel. However, the anti-cancer effects and the molecular mechanisms of tectochrysin in colon cancer cells have not yet been reported. We investigated whether tectochrysin could inhibit colon cancer cell growth at 1, 5, 10 μg/ml. In in vivo study, we injected a tectochrysin treatment dose of 5 mg/kg to each mouse. Tectochrysin suppressed the growth of SW480 and HCT116 human colon cancer cells. The expression of DR3, DR4 and Fas were significantly increased, and pro-apoptotic proteins were also increased. Tectochrysin treatment also inhibited activity of NF-κB. A docking model indicated that tectochrysin binds directly to the p50 unit. In in vivo, tumor weights and volumes in mice were reduced when treated with tectochrysin. Tectochrysin leads to apoptotic cell death in colon cancer cells through activation of death receptors expression via the inhibition of NF-κB. Tectochrysin can be a useful agent for the treatment of colon cancer cell growth as well as an adjuvant agent for chemo-resistant cancer cells growth.
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影响因子：
5.1
作者：
Hu, Weicheng;Shen, Ting;Wang, Myeong-Hyeon
通讯作者：
Wang, Myeong-Hyeon
影响因子：
12.8
作者：
Kang, You Jung;Kim, In Young;Choi, Kyeong Sook
通讯作者：
Choi, Kyeong Sook
影响因子：
7.3
作者：
Kollipara, Pushpa Saranya;Jeong, Heon Sang;Hong, Jin Tae
通讯作者：
Hong, Jin Tae
影响因子：
7.2
作者：
Ban, Jung Ok;Jung, Young-Suk;Hong, Jin Tae
通讯作者：
Hong, Jin Tae
DOI：
10.1152/ajpgi.00248.2006
发表时间：
2007-01-01
影响因子：
4.5
作者：
Lim, Do Y.;Jeong, Yoonhwa;Park, Jung H. Y.
通讯作者：
Park, Jung H. Y.