The effect of surface pre‐reacted glass‐ionomer filler eluate on dental pulp cells and mineral deposition on dentin: In vitro study
The effect of surface pre‐reacted glass‐ionomer filler eluate on dental pulp cells and mineral deposition on dentin: In vitro study
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表面预反应玻璃离子填料洗出液对牙髓细胞和牙本质矿物质沉积的影响：体外研究
DOI：
10.1111/eos.12777
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发表时间：
2021
影响因子：
1.9
通讯作者：
Tagami Junji
中科院分区：
文献类型：
--
作者：
Nishimaki Mayuri;Nassar Mohannad;Tamura Yukihiko;Hiraishi Noriko;Dargham Ahmad;Nikaido Toru;Tagami Junji
研究了表面预反应玻璃离子（S-PRG）填充剂对牙髓细胞和牙本质沉积物组成的影响。在用不同浓度的S-PRG填充剂处理牙髓细胞后6小时和24小时进行增殖（CCK-8）、细胞毒性（LDH）和分化活性（ALP）测试，沿着细胞形态观察。将牙本质表面浸泡在去离子水或S-PRG填充剂中，然后浸泡在去离子水或模拟体液中，并在扫描电子显微镜下观察和使用能量色散X射线光谱仪进行元素分析。24 h时，各组间CCK-8和ALP活性值存在显著差异，且呈浓度依赖性。各组间LDH检测数据无显著性差异。细胞形态在两个暴露时间均未发生改变。然而，在最高浓度下观察到细胞密度降低。在浸入S-PRG填充剂中的样品中观察到结晶沉积物和牙本质小管闭塞，随后浸入模拟体液中，与最初未用S-PRG填充剂处理的表面相比，模拟体液中的某些离子浓度也较高。最低的两个浓度的三氯甲烷没有显示出显着的毒性。S-PRG增强了模拟体液在矿物沉积物形成中的作用。
The effects of surface pre‐reacted glass‐ionomer (S‐PRG) filler on pulpal cells and on the composition of dentinal deposits were investigated. Proliferation (CCK‐8), cytotoxicity (LDH), and differentiation activity (ALP) tests, along with cell morphology observations, were conducted at 6 and 24 h after treatment of pulpal cells with different S‐PRG filler eluate concentrations. Dentinal surfaces were immersed in deionized water or S‐PRG filler eluate followed by immersion in deionized water or simulated body fluid and observed under scanning electron microscope and elemental analysis using energy dispersive x‐ray spectrometer. At 24 h, there were significant differences in CCK‐8 and ALP activity values between the groups in a concentration‐dependent manner. LDH test data were not significantly different among the groups. Cell morphology was not altered at either exposure time. However, decreased cellular density was observed with the highest eluate concentration. Crystalline deposits and occluded dentinal tubules were observed in samples immersed in S‐PRG filler with a later immersion in simulated body fluid, which also showed higher concentrations of certain ions compared to surfaces that were not initially treated with S‐PRG filler. The lowest two eluate concentrations did not show significant toxicity. S‐PRG enhanced the effect of simulated body fluid in the formation of mineral deposits.