Kisspeptin Receptor, GPR54, as a Candidate for the Regulation of Testicular Activity in the Frog Rana esculenta
Kisspeptin Receptor, GPR54, as a Candidate for the Regulation of Testicular Activity in the Frog Rana esculenta
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DOI：
10.1095/biolreprod.112.103515
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发表时间：
2013-03-01
影响因子：
3.6
通讯作者：
Meccariello, Rosaria
中科院分区：
文献类型：
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作者：
Chianese, Rosanna;Ciaramella, Vincenza;Meccariello, Rosaria
Kisspeptins通过GPR 54起作用，是控制生殖轴的新参与者。它们能够与GnRH神经元通信，发送环境，代谢和性腺信号，最终诱导GnRH和LH分泌。目前，对kisspeptin信号在性腺中的生理意义的研究很少。我们克隆了GPR 54受体的无尾两栖动物蛙精巢，并研究了其在几个组织（脑，脊髓，卵巢，肌肉和肾脏）的表达。特别地，在年度性周期期间在垂体和睾丸中进行表达分析。在冬季停滞期（2月）结束时，Pestival和睾丸GPR 54 mRNA增加，并在繁殖季节（4月）达到高水平。通过原位杂交分析GPR 54在睾丸中的表达，发现GPR 54存在于间质区室和增殖的生殖细胞中。睾丸GPR 54表达在2月和6月被指示为雌二醇依赖。此外，在2月，kisspeptin-10（Kp-10）以剂量依赖性方式诱导GPR 54和雌激素受体α（ER α）的睾丸表达。相反，在3月，Kp-10对ER α的表达有双相作用，在短时间（1小时）内抑制，在较长时间（4小时）内刺激。总之，我们的研究结果表明，青蛙睾丸表达GPR 54在雌二醇依赖的方式和Kp-10调节睾丸表达的ER α，因此，kisspeptin/GPR 54系统可能是局部参与调节雌激素依赖的睾丸功能，如生殖细胞增殖和类固醇生成。
Kisspeptins, acting via GPR54, are new players in the control of reproductive axis. They have the ability to communicate with GnRH neurons sending environmental, metabolic, and gonadal signals, with the induction of GnRH and LH secretion as final effect. At present, the physiological significance of kisspeptin signaling in the gonad is poorly investigated. We cloned GPR54 receptor from the anuran amphibian Rana esculenta testis and investigated its expression in several tissues (brain, spinal cord, ovary, muscle, and kidney). In particular, the expression analysis was carried out in pituitary and testis during the annual sexual cycle. Pituitary and testicular GPR54 mRNA increased at the end of the winter stasis (February) and reached high levels during the breeding season (April). The analysis of GPR54 expression in testis was reinforced by in situ hybridization that revealed GPR54 presence in the interstitial compartment and in proliferating germ cells. Testicular GPR54 expression in February and in June was indicated to be estradiol dependent. Furthermore, in February, kisspeptin-10 (Kp-10) induced the testicular expression of both GPR54 and estrogen receptor alpha (ERalpha) in a dose-dependent manner. Conversely, in March, Kp-10 had a biphasic effect on the expression of ERalpha, being inhibitory at short (1 h) and stimulatory at longer (4 h) incubation time. In conclusion, our results demonstrate that frog testis expresses GPR54 in an estradiol-dependent manner and that Kp-10 modulates the testicular expression of ERalpha; thus, the kisspeptin/GPR54 system might be locally involved in the regulation of estrogen-dependent testicular functions such as germ cell proliferation and steroidogenesis.