Mouse out root sheath cells cultured on E-cadherin-coated dishes exhibit neuronal cell morphology
Mouse out root sheath cells cultured on E-cadherin-coated dishes exhibit neuronal cell morphology
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在 E-钙粘蛋白包被的培养皿上培养的小鼠根鞘细胞表现出神经元细胞形态
DOI：
10.1002/apj.467
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发表时间：
2011-11
影响因子：
1.8
通讯作者：
Lingling Xia, Qing Liu,Wei Liu
中科院分区：
文献类型：
--
作者：
Lingling Xia, Qing Liu,Wei Liu
为了更好地维持干细胞的自我更新功能和细胞增殖潜能，上皮钙粘蛋白（E-cadherin）包被系统被用于培养胚胎未分化细胞。本研究以10 μg/ml的重组E-cad-Fc包被培养皿，观察其对培养的小鼠外根鞘（ORS）细胞行为的影响。结果表明，在E-cadherin包被的培养皿上培养ORS细胞可导致部分ORS细胞发生神经元细胞形态学改变。此外，与未包被组相比，包被组Nestin基因表达上调。特别地，具有连接到附近细胞的细细胞突起的伸长的细胞形态在强烈粘附到涂覆的培养皿的细胞亚群中变得显著。这些结果初步表明E-cadherin包被可能有利于毛囊源性细胞的神经源性分化，值得进一步深入研究。© 2010科廷科技大学和John Wiley & Sons有限公司。
Epithelial cadherin (E-cadherin) coating system has been employed to culture embryonic undifferentiated cells in order to better maintain stem-cell self-renewal function and cell proliferation potential. In this study, recombinant E-cad-Fc was used to coat the dish at a concentration of 10 µg/ml to observe the effect of the coating on cell behavior of cultured mouse out root sheath (ORS) cells. The results demonstrated that culturing the ORS cells on E-cadherin-coated dishes could lead to neuronal cell morphology change of part of the ORS cells. In addition, Nestin gene expression was upregulated in the cells of coated group compared to the cells of non-coated group. Particularly, elongated cell morphology with fine cell processes connecting to nearby cells became significant in the subpopulation of the cells that strongly adhered to the coated culture dish. These results preliminarily indicate that E-cadherin coating may favor the neurogenic lineage differentiation of hair follicle derived cells, which deserves further detailed studies. © 2010 Curtin University of Technology and John Wiley & Sons, Ltd.
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DOI：
10.1016/s0093-3619(08)70911-0
发表时间：
2008
期刊：
Yearbook of Dermatology and Dermatologic Surgery
影响因子：
--
作者：
B. Thiers
通讯作者：
B. Thiers
影响因子：
3.7
作者：
Kloepper, J. E.;Hendrix, S.;Paus, R.
通讯作者：
Paus, R.
DOI：
10.1002/bdrc.20008
发表时间：
2004-06-01
期刊：
Birth defects research. Part C, Embryo today : reviews
影响因子：
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作者：
Sieber-Blum, Maya;Grim, Milos
通讯作者：
Grim, Milos
DOI：
10.1073/pnas.0501263102
发表时间：
2005-04-12
影响因子：
11.1
作者：
Amoh, Y;Li, L;Hoffman, RM
通讯作者：
Hoffman, RM
DOI：
10.1093/proeng/gzg037
发表时间：
2003-04-01
期刊：
PROTEIN ENGINEERING
影响因子：
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作者：
Nagaoka, M;Akaike, T
通讯作者：
Akaike, T