"In vitro" protection of DNA from Fenton reaction by plant polyphenol verbascoside.
"In vitro" protection of DNA from Fenton reaction by plant polyphenol verbascoside.
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DOI：
10.1016/j.bbagen.2005.02.004
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发表时间：
2005-05
期刊：
影响因子：
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通讯作者：
Chenyang Zhao;G. Dodin;Chenshan Yuan;Haifeng Chen;R. Zheng;Z. Jia;B. Fan
中科院分区：
文献类型：
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作者：
Chenyang Zhao;G. Dodin;Chenshan Yuan;Haifeng Chen;R. Zheng;Z. Jia;B. Fan
The protection effect of verbascoside (Ver) against Fenton reaction on plasmid pBR322 DNA was studied using agarose gel electrophoresis and UV-visible spectroscopy. The pBR322 plasmid DNA is damaged by hydroxyl radical (OH) generated from the Fenton reaction with H2O2and Fe(II) or Fe(III). This DNA damage is characterized by the diminution of supercoiled DNA forms or by the increase of relaxed or linear DNA forms after oxidative attack. The UV spectrum study showed that verbascoside can form complexes with Fe(II) or Fe(III), and the complexation can be reversed by the addition of EDTA. The formation constants of verbascoside–Fe complexes were estimated as 1021.03and 1031.94M−2for Fe(II) and Fe(III) respectively. The inhibition of Fenton reaction by verbascoside could be partially explained by the sequestration of Fe ions.
