3D-printed biodegradable gyroid scaffolds for tissue engineering applications
3D-printed biodegradable gyroid scaffolds for tissue engineering applications
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DOI：
10.1016/j.matdes.2018.04.037
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发表时间：
2018-08-05
影响因子：
8.4
通讯作者：
Dupont-Gillain, Christine
中科院分区：
文献类型：
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作者：
Germain, Loic;Fuentes, Carlos A.;Dupont-Gillain, Christine
熔融沉积成型技术是一种低成本、易使用的添加剂制造技术，可用于制备聚乳酸回旋支架。选择这种形态是因为它的弹簧形状，高孔隙率，导致良好的营养和废物扩散，以及良好的机械性能。对印刷参数进行了优化，证明需要一种载体材料来改善印刷。将陀螺仪与普通的支撑式结构进行了比较。通过Micro-CT测量支架孔隙率，通过压缩试验确定力学性能，考虑了几何形状、印刷分辨率和聚乳酸结晶度的影响。研究了支架的几何形状和结晶度对其体外降解的影响。与打印模型预期的一样，陀螺结构的孔隙率为71%。压缩试验表明，与支撑基支架相比，陀螺体具有各向同性的特性。在生理条件下老化后，陀螺形支架在周期间保持其完整性，而对照支架从第33周开始失去支柱，其方式取决于结晶度和印刷分辨率。基于这些结果，提出了一种适合于组织工程支架的网状结构设计，该结构可以利用有限差分技术进行细化，以生产低成本和个性化的植入物。(C)2018爱思唯尔有限公司。保留所有权利。
Fused deposition modeling (FDM), a low-cost and easy-to-use additive manufacturing technique, was used to produce poly(lactic acid) (PLA) gyroid scaffolds. Such morphology was selected for its spring shape, high porosity leading to good nutrient and waste diffusion, and favorablemechanical properties. Printing parameters were optimized and the need of a support material to improve printing was evidenced. The gyroid was compared to the common strut-based structure. Scaffold porosity was measured by micro-CT, and mechanical properties were determined by compression tests, taking into account the effect of geometry, printing resolution, and PLA crystallinity. The impact of scaffold geometry and crystallinity on its degradation was studied in vitro. Porosity of the gyroid structure was 71%, as expected from the printing model. The compression tests showed an isotropic behavior for the gyroid, in contrast with the strut-based scaffold. Upon aging in physiological conditions, gyroid scaffolds retained their integrity during 64 weeks, while control scaffolds lost struts starting from week 33, in a way that depended on crystallinity and printing resolution. Based on these results, the gyroid design is proposed as a suitable mesh architecture for tissue engineering scaffolds that can be elaborated using FDM techniques, to produce low-cost and personalized implants. (C) 2018 Elsevier Ltd. All rights reserved.