Photoperiod affects the expression of neural cell adhesion molecule and polysialic acid in the hypothalamus of the Siberian hamster.
Photoperiod affects the expression of neural cell adhesion molecule and polysialic acid in the hypothalamus of the Siberian hamster.
复制标题
光周期影响西伯利亚仓鼠下丘脑神经细胞粘附分子和聚唾液酸的表达。
DOI：
10.1016/0006-8993(95)00588-h
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Glass,JD
中科院分区：
文献类型：
--
作者：
Lee,W;Watanabe,M;Glass,JD
采用免疫组织化学和免疫印迹方法检测抑制性短日照（SD）光周期对成年雄性西伯利亚仓鼠下丘脑和视前区（POA）神经细胞粘附分子（NCAM）及其多唾液酸化形式（PSA-NCAM）表达的影响。在经历了 SD 诱导的性腺退化的动物中，免疫印迹分析显示下丘脑前部 (AH) 和下丘脑基底内侧 (MBH) 区域的免疫反应性 PSA 含量显着降低。这些变化伴随着 POA 和 AH 中 180 kDa NCAM 同工型含量的增加，以及 MBH 中含量的减少。 AH 中 140 kDa NCAM 亚型也升高。光学显微镜分析显示，暴露于 SD 的动物 MBH 中 NCAM 免疫反应性 tanycyte 样过程的密度显着降低。这种效应并没有被去势所阻断，表明这可能是下丘脑光周期改变的主要（与性类固醇无关的）效应。此外，在 SD 暴露下保持睾丸活跃的无反应仓鼠中，光周期诱导的 NCAM 表达变化并不明显。总的来说，这些结果证明光周期的季节变化会影响下丘脑中 NCAM 和 PSA 的表达。这些变化可能有助于促进与季节性生殖和/或代谢周期调节相关的可塑形态重排。
Immunohistochemical and immunoblot procedures were used to examine the effects of inhibitory short day (SD) photoperiod on the expression of neural cell adhesion molecule (NCAM) and its polysialylated form (PSA-NCAM) in the hypothalamus and preoptic area (POA) of the adult male Siberian hamster. In animals that had undergone SD-induced gonadal regression, immunoblot analyses revealed significant reductions in the content of immunoreactive PSA in anterior hypothalamic (AH) and mediobasal hypothalamic (MBH) regions. These changes were accompanied by increased contents of the 180 kDa NCAM isoform in the POA and AH, and decreased content in the MBH. The 140 kDa NCAM isoform also was elevated in the AH. Light microscopic analysis revealed a marked reduction in the density of NCAM-immunoreactive tanycyte-like processes in the MBH of animals exposed to SD. This effect was not blocked by castration, indicating that this may be a primary (sex steroid-independent) effect of altered photoperiod in the hypothalamus. Also, photoperiod-induced alterations in NCAM expression were not evident in non-responsive hamsters that maintained active testes under SD exposure. Collectively, these results are evidence that seasonal changes in photoperiod affect the expression of NCAM and PSA in the hypothalamus. Such changes could help promote plastic morphological rearrangements related to the regulation of seasonal reproductive and/or metabolic cycles.