Mining a yeast library for brain endothelial cell-binding antibodies
Mining a yeast library for brain endothelial cell-binding antibodies
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DOI：
10.1038/nmeth993
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发表时间：
2007-02-01
期刊：
影响因子：
48
通讯作者：
Shusta, Eric V.
中科院分区：
文献类型：
--
作者：
Wang, Xin Xiang;Cho, Yong Ku;Shusta, Eric V.
We describe the use of yeast surface display for the identification of antibodies that bind the plasma membranes of living cells. Yeast panning with a nonimmune human single-chain antibody library identified 34 unique lead antibodies that bind (K-d = 82 +/- 15 nM) and in some cases internalize into rat brain endothelial cells. In addition, we used a new yeast display immunoprecipitation procedure for initial characterization of the cognate antigens.
