Efficacy of an MPC-BMA co-polymer as a nanotransporter for paclitaxel.
Efficacy of an MPC-BMA co-polymer as a nanotransporter for paclitaxel.
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发表时间：
2007-05
影响因子：
2
通讯作者：
M. Wada;H. Jinno;M. Ueda;T. Ikeda;M. Kitajima;T. Konno;J. Watanabe;K. Ishihara
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文献类型：
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作者：
M. Wada;H. Jinno;M. Ueda;T. Ikeda;M. Kitajima;T. Konno;J. Watanabe;K. Ishihara
背景技术紫杉醇(PTX)由于其低水溶性而以聚氧乙烯化蓖麻油(CO)中的溶液形式施用，但溶剂引起的副作用可能很严重。材料和方法 PMB30W 是 2-甲基丙烯酰氧基乙基磷酰胆碱 (MPC) 和甲基丙烯酸丁酯 (BMA) 的共聚物。在细胞培养物和体内检查了 PMB30W (PTX-PMB30W) 中 PTX 的细胞毒性。结果 PTX-PMB30W 和二甲基亚砜中的 PTX 在乳腺癌细胞系 MCF-7、SK-BR-3 和 MX-1 中显示出相似的毒性。在移植 MX-1 细胞的裸鼠中每周腹腔注射 50 mg/kg PTX 后，PTX-PMB30W 和 PTX in CO (PTX-CO) 的抗肿瘤功效相似。在 200 mg/kg PTX 下，所有动物在 PTX-CO 给药后 1 分钟内死亡。然而，所有接受 PTX-PBM30W 的动物都存活了下来。皮下注射PTX-CO后出现溃疡，但注射PTX-PMB30W并未引起皮肤变化。结论 我们的数据表明 PMB30W 可以作为有效的 PTX 纳米转运蛋白。
BACKGROUND Paclitaxel (PTX) is administered as a solution in polyoxyethylated castor oil (CO) due to its low water solubility, but solvent-induced side-effects may be severe. MATERIALS AND METHODS PMB30W is a co-polymer of 2-methacryloyloxyethyl phosphorylcholine (MPC) and butyl methacrylate (BMA). Cytotoxicities of PTX in PMB30W (PTX-PMB30W) were examined in cell culture and in vivo. RESULTS PTX-PMB30W and PTX in dimethyl sulfoxide showed similar toxicity in breast cancer cell lines MCF-7, SK-BR-3 and MX-1. Antitumor efficacies of PTX-PMB30W and PTX in CO (PTX-CO) were similar following weekly intraperitoneal administration of 50 mg/kg PTX in nude mice transplanted with MX-1 cells. At 200 mg/kg PTX, all animals died within 1 minute of PTX-CO administration. However, all animals receiving PTX-PBM30W survived. Ulceration occurred following subcutaneous injection of PTX-CO, but injection of PTX-PMB30W did not cause skin changes. CONCLUSION Our data suggest that PMB30W can act as an effective PTX nanotransporter.