The germ plasm component Vasa allows tracing of the interstitial stem cells in the cnidarian Hydractinia echinata
The germ plasm component Vasa allows tracing of the interstitial stem cells in the cnidarian Hydractinia echinata
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DOI：
10.1002/dvdy.21562
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发表时间：
2008-06-01
影响因子：
2.5
通讯作者：
Plickert, G.
中科院分区：
文献类型：
--
作者：
Rebscher, Nicole;Volk, C.;Plickert, G.
水螅体中的生殖细胞终生来源于多能间质干细胞。要发现，如果一个真正的生殖细胞体分离存在于这些物种中，我们研究了配子发生在海胆生殖细胞标记Vasa使用原位杂交和免疫组织化学。我们可以证明Hevas是一种合子转录物，从原肠期开始就存在于胚胎中。在浮游幼虫中，Hevas在位于内胚层的间质干细胞中表达。在变态期间，表达Heva的细胞出现在息肉下半部的外胚层中。虽然Hevas转录本在发育中的配子中检测不到，但该蛋白在卵子发生过程中积累。受精后，在极体形成极可检测到含有颗粒的导管。这些结果表明，在Hydroactinia，母体Vasa蛋白，但不是mRNA，是一个母体的种质成分，并可能参与间质干细胞的规格和维护。
Germ cells in hydrozoans arise lifelong from multipotent interstitial stem cells. To discover if a true germline-soma segregation exists in these species, we studied gametogenesis in Hydractinia echinata using in situ hybridization and immunohistochemistry for the germ cell marker Vasa. We could show that Hevas is a zygotic transcript, present in embryos from the gastrula stage onward. In the planula larva, Hevas is expressed in the interstitial stem cells located in the endoderm. During metamorphosis, Hevas-expressing cells appear in the ectoderm in the lower half of the polyp. While the Hevas transcript is not detectable in developing gametes, the protein accumulates during oogenesis. Vasa containing granules are detectable at the polar-body-forming pole after fertilization. These results suggest that, in Hydractinia, maternal Vasa protein, but not the mRNA, is a maternal constituent of a germ plasm and might be involved in the specification and maintenance of interstitial stem cells.