First con firmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community
First con firmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community
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DOI：
10.1016/j.scitotenv.2020.138764
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发表时间：
2020-08-01
影响因子：
9.8
通讯作者：
Mueller, Jochen F.
中科院分区：
文献类型：
--
作者：
Ahmed, Warish;Angel, Nicola;Mueller, Jochen F.
SARS-CoV-2（当前的 COVID-19 大流行的病原体）的感染伴随着粪便中病毒的排出。因此，废水中 SARS-CoV-2 的定量提供了通过基于废水的流行病学 (WBE) 监测人群中感染流行情况的能力。在目前的工作中，从澳大利亚一个流域的废水中浓缩了 SARS-CoV-2 RNA，并使用逆转录酶定量聚合酶链反应 (RT-qPCR) 计数了病毒 RNA 拷贝，结果在六天内从同一废水处理厂 (WWTP) 检测到两次阳性。然后，通过蒙特卡罗模拟，利用在废水中观察到的估计病毒 RNA 拷贝数来估计流域内受感染个体的数量。考虑到输入参数的不确定性和变化，该模型估计流域内感染者的中位数范围为 171 至 1,090 人，这与临床观察结果相当一致。这项工作强调了 WBE 在社区中监测传染病（例如 COVID-19）的可行性。这项工作还引起人们的注意，需要对废水中的包膜病毒进行进一步的方法学和分子测定验证。
Infection with SARS-CoV-2, the etiologic agent of the ongoing COVID-19 pandemic, is accompanied by the shedding of the virus in stool. Therefore, the quantification of SARS-CoV-2 in wastewater affords the ability to monitor the prevalence of infections among the population via wastewater-based epidemiology (WBE). In the current work, SARS-CoV-2 RNA was concentrated from wastewater in a catchment in Australia and viral RNA copies were enumerated using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) resulting in two positive detections within a six day period from the same wastewater treatment plant (WWTP). The estimated viral RNA copy numbers observed in the wastewater were then used to estimate the number of infected individuals in the catchment via Monte Carlo simulation. Given the uncertainty and variation in the input parameters, the model estimated a median range of 171 to 1,090 infected persons in the catchment, which is in reasonable agreement with clinical observations. This work highlights the viability of WBE for monitoring infectious diseases, such as COVID-19, in communities. The work also draws attention to the need for further methodological and molecular assay validation for enveloped viruses in wastewater.