Bioinspired antibacterial surface for orthopedic and dental implants
Bioinspired antibacterial surface for orthopedic and dental implants
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DOI：
10.1002/jbm.b.34762
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发表时间：
2020-11-25
影响因子：
3.4
通讯作者：
Dua, Rupak
中科院分区：
文献类型：
--
作者：
Elliott, Drew T.;Wiggins, Russell J.;Dua, Rupak
细菌感染仍然是骨科和牙科种植体失败的一个重要问题。以前的研究集中在修改表面纹理，粗糙度，或涂层植入物与抗生素，以提供增强的抗菌性能。然而，它们的成功有限。在这项研究中，我们试图工程钛（Ti）合金表面仿生纳米水平上使用碱性水热处理（AHT）的启发，翅膀的结构。在230 ℃下使用4和8小时AHT对钛板的两个改性表面进行显影。我们发现对照板显示出相对光滑的表面，表面上几乎没有伪影。相比之下，4小时AHT和8小时AHT板显示高度分别约为250-350和100- 1，250 nm的纳米尖峰，其随机分布在整个表面上。我们发现两种S.金黄色葡萄球菌和铜绿假单胞菌细菌菌株在动态环境中孵育1小时时与对照组相比。AHT 8小时组比对照组多杀38.97%。金黄色葡萄球菌在静置培养中的抑菌率为11.27%，在动态环境中的抑菌率为11.27%。总的来说，研究结果表明，AHT在钛上产生的纳米结构显示出显著的杀菌特性。因此，我们建议在未来的骨科和牙科金属植入物表面进行碱性水热处理。
Bacterial infections still present a significant concern in orthopedic and dental implant failure. Previous investigations have focused on modifying the surface texture, roughness, or coating implants with antibiotics to provide enhanced anti-bacterial properties. However, they have demonstrated limited success. In this study, we attempted to engineer the titanium (Ti) alloy surface biomimetically at the nano level using alkaline hydrothermal treatment (AHT) inspired by cicada's wing structure. Two modified surfaces of Ti plates were developed using 4 and 8-hr AHT at 230 degrees C. We found that the control plates showed a relatively smooth surface, with little artifacts on the surface. In contrast, 4-hr AHT and 8-hr AHT plates showed nano-spikes of heights around 250-350 and 100-1,250 nm, respectively, that were distributed randomly all over the surface. We found a statistically significant (p < 0.05) number of non-viable cells for both S. aureus and P. aeruginosa bacterial strains when incubated for 1 hr in a dynamic environment when compared with the control group. The 8-hr AHT groups killed 38.97% more S. aureus in static culture and 11.27% in a dynamic environment than the 4-hr AHT. Overall, the findings indicate that the nanostructures generated on titanium by the AHT showed significant bactericidal properties. We, therefore, recommend conducting alkaline hydrothermal treatment on the surfaces for future orthopedic and dental metallic implants.