Isolation and Identification of Lactic Acid Bacteria Inhibiting Gastro-intestinal Pathogenic Bacteria of Domestic Animal.
Isolation and Identification of Lactic Acid Bacteria Inhibiting Gastro-intestinal Pathogenic Bacteria of Domestic Animal.
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发表时间：
2002
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通讯作者：
Lee;Jae Yeon;K. Hwang;Hyun Su Kim;Keun P. Kim;S. I. Sung
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作者：
Lee;Jae Yeon;K. Hwang;Hyun Su Kim;Keun P. Kim;S. I. Sung
水原邮政局Box 77，Konggido 440-600，韩国-为了分离对动物胃肠道致病菌如鸡沙门氏菌、金黄色葡萄球菌和大肠杆菌具有上级抑制活性的益生乳酸菌，最初从韩国本地鸡的小肠中分离130株，最终选择7株乳酸菌。通过API CHL试剂盒和16 S rRNA序列测定，筛选出的乳酸菌除BD 14为戊糖片球菌外，其余均为乳杆菌属。其中戊糖乳杆菌K34对盐酸和胆盐的耐受性最好，对沙门氏菌的抑制活性也最高。鸡S. aureus和E.杆菌所选菌株对新霉素、红霉素、头孢菌素、阿莫西林/克拉维酸、氨苄西林、土霉素等多种抗生素敏感，对环丙沙星耐药。除BL株外，其余菌株均对粘菌素和链霉素耐药，BD 14、BD 16、K34株对庆大霉素耐药。
Suwon P.O. Box 77, Kyounggido 440-600, Korea - To isolate probiotic lactic acid bacteria having superior inhibitory activities against animal gastro-intestinal pathogenic bacteria such as Salmonella gallinarum, Staphylococcus aureus and Escherichia coli, 130 strains were initially isolated from the small intestines of Korean native chickens and 7 lactic acid bacteria were finally selected. By using API CHL kit and 16S rRNA sequencing method, the selected lactic acid bacteria were found to be belonged to genus Lactobacillus except BD14 identified as Pediococcus pentosaceus. Especially, Lactobacillus pentosus K34 showed the highest resistancy to both of HCl and bile salt, as well as the highest inhibitory activities against S. gallinarum, S. aureus and E. coli. All the selected strains were sensitive to various antibiotics such as neomycin, erythromycin, cephalosporin, amoxicillin/clavulanic acid, ampicillin, oxytetracycline, but resistant to ciprofloxacin. All the selected strains except BL strain were resistant to colistin and streptomycin, and BD14, BD16, K34 strains were resistant to gentamicin.