Microarchitectural analysis of decellularised unscarred and scarred dermis provides insight into the organisation and ultrastructure of the human skin with implications for future dermal substitute scaffold design
Microarchitectural analysis of decellularised unscarred and scarred dermis provides insight into the organisation and ultrastructure of the human skin with implications for future dermal substitute scaffold design
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DOI：
10.1177/2041731419843710
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发表时间：
2019-05-01
影响因子：
8.2
通讯作者：
Bayat, Ardeshir
中科院分区：
文献类型：
--
作者：
Khan, Umair;Bayat, Ardeshir
真皮组织的三维空间排列在伤口愈合过程中指导细胞行为方面起着至关重要的作用。这是至关重要的阐明更好地了解人体皮肤的三维真皮结构。我们试图了解无瘢痕皮肤和正常营养性瘢痕之间的脱细胞人真皮的形态结构的配置。皮肤活检进行脱细胞化（DNA去除= 88%）。组织学分析显示脱细胞无瘢痕和瘢痕真皮的大体形态没有变化。多光子和原子力显微镜显示，无瘢痕脱细胞真皮中的胶原纤维交织在一起，类似于网状结构。脱细胞无瘢痕真皮中的胶原纤维硬度较低（平均值：2.155 +/- 0.9595 MPa; p < 0.0001），表面形貌较粗糙（R-q = 16.5，R-a = 12.5，R-max = 198; p < 0.0001）。瘢痕真皮具有较高的胶原体积密度（乳头状真皮，p < 0.0082;网状真皮，p < 0.0332）。结果表明，支架应表现出具有仿生表面和低刚度的网状结构。
The three-dimensional spatial arrangement of dermal tissue plays a crucial role in directing cellular behaviour during wound healing. It is vital to elucidate a better understanding of the three-dimensional dermal architecture of human skin. We sought to understand the configuration in morphological structure of decellularised human dermis between unscarred skin and normotrophic scars. Skin biopsies underwent decellularisation (DNA removal = 88%). Histological analysis showed no change in gross morphology of decellularised unscarred and scarred dermis. Multiphoton and atomic force microscopies showed that collagen fibres in unscarred decellularised dermis were interwoven akin to a mesh-like structure. Collagen fibres in decellularised unscarred dermis were less stiff (mean: 2.155 +/- 0.9595 MPa; p < 0.0001) with a rougher (R-q = 16.5, R-a = 12.5, R-max = 198; p < 0.0001) surface topography. Scarred dermis had a higher collagen volume density (papillary dermis, p < 0.0082; reticular dermis, p < 0.0332). The results demonstrate that scaffolds should exhibit a mesh-like structure with a biomimetic surface and low stiffness.