Fabrication of topologically complex three-dimensional microfluidic systems in PDMS by rapid prototyping
Fabrication of topologically complex three-dimensional microfluidic systems in PDMS by rapid prototyping
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DOI：
10.1021/ac9912294
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发表时间：
2000-07-15
影响因子：
7.4
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Anderson, JR;Chiu, DT;Whitesides, GM
本文描述了一种在聚二甲基硅氧烷(PDMS)中制作拓扑复杂的三维微流控通道系统的过程，该过程被称为“膜夹层”方法，以建议最终系统的结构：在两个较厚的平板之间夹着提供结构支撑的两个较厚的平板之间的薄膜，在每一面上模制通道结构(以及面与面之间的连接)。采用两级光刻和复制模塑的快速成型技术制造了两个“母版”。它们在压力下面对面排列，中间有PDMS预聚体。PDMS是热固化的，母版具有互补的对准轨迹，因此配准很简单。所得到的薄的PDMS膜可以通过一次一个地移除两个母片的一系列步骤转移和密封到另一膜或PDMS板上；这些步骤发生时不会使特征变形。这种方法可以制造包含通道的膜，该通道在其自身和下方交叉，但不自相交，因此可以以任何结的形式被制造。由此得出，该方法可以产生拓扑复杂的微流体系统；这种能力被制造为“篮子编织”结构。通过填充通道和去除膜，可以制造复杂的微结构。堆叠和密封多个膜允许比在一个膜中可能的更复杂的几何形状。环绕但不连接直通道的方形盘绕通道说明了这种类型的复杂性。
This paper describes a procedure for making topologically complex three-dimensional microfluidic channel systems in poly(dimethylsiloxane) (PDMS), This procedure is called the "membrane sandwich" method to suggest the structure of the final system: a thin membrane having channel structures molded on each face (and with connections between the faces) sandwiched between two thicker, flat slabs that provide structural support. Two "masters" are fabricated by rapid prototyping using two-level photolithography and replica molding. They are aligned face to face, under pressure, with PDMS prepolymer between them. The PDMS is cured thermally, The masters have complementary alignment tracks, so registration is straightforward. The resulting, thin PDMS membrane can be transferred and sealed to another membrane or slab of PDMS by a sequence of steps in which the two masters are removed one at a time; these steps take place without distortion of the features. This method can fabricate a membrane containing a channel that crosses over and under itself, but does not intersect itself and, therefore, can be fabricated in the form of any knot, It follows that this method can generate topologically complex microfluidic systems; this capability is demonstrated by the fabrication of a "basketweave" structure. By filling the channels and removing the membrane, complex microstructures can be made. Stacking and sealing more than one membrane allows even more complicated geometries than are possible in one membrane. A square coiled channel that surrounds, but does not connect to, a straight channel illustrates this type of complexity.