Increase of oxidative stress in human sperm with lower motility
Increase of oxidative stress in human sperm with lower motility
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DOI：
10.1016/j.fertnstert.2007.05.029
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发表时间：
2008-05-01
影响因子：
6.7
通讯作者：
Wei, Yau-Huei
中科院分区：
文献类型：
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作者：
Kao, Shu-Huei;Chao, Hsiang-Tai;Wei, Yau-Huei
目的：探讨氧化应激状态对人类精子活力的影响。设计：证实氧化损伤程度越高的精子抗氧化能力越低。地点：大学医院不育中心。患者(S)：来自35名精液性状正常的健康捐精者和43名不育或不育男性的精液样本。干预(S)：氧化损伤水平(8-羟基-2‘-脱氧鸟苷和过氧化脂质)和抗氧化剂(视黄醇、α-生育酚、抗坏血酸、主要观察指标(S)：分别采用高效液相-电化学检测法和高效液相-荧光检测法分析精子和/或精浆中8-羟色胺和过氧化脂质的比含量。维生素C和α-生育酚的分析采用高效液相色谱法，抗坏血酸和蛋白硫醇的测定采用分光光度法。8-羟基-2‘-脱氧鸟苷用抗8-OHdG抗体与异硫氰酸荧光素偶联，免疫荧光染色显示。用荧光染料C11-BODIPY581/591对精子的过氧化脂质进行染色。结果：精子活力与8-羟色胺呈显著负相关，与过氧化脂质呈显著负相关(S)。精子活力与精浆中视黄醇、α-生育酚、抗坏血酸和蛋白硫醇水平呈显著正相关。精子中的8-羟基-2‘-脱氧鸟苷和过氧化脂质主要存在于线粒体中。结论：不育或不育男性精子活力下降时，氧化应激和氧化损伤显著增加，精子和精浆中的抗氧化能力降低。
Objective: To investigate the causal role of oxidative-stress status on human sperm motility.Design: To demonstrate that sperm with higher oxidative damage have a lower antioxidant capacity.Setting: University hospital infertility center.Patient(s): Seventy-eight semen samples were obtained from 35 healthy donors who had normal semen characteristics and from 43 infertile or subfertile males.Intervention(S): The levels of oxidative damage (8-hydroxy-2'-deoxyguanosine [8-OHdG] and lipid peroxides) and antioxidants (retinol, a-tocopherol, ascorbate, and protein thiols) in the spermatozoa and/or seminal plasma were measured.Main Outcome Measure(s): We analyzed the specific content of 8-OHdG and lipid peroxides by using high-performance liquid chromatography (HPLC)-electrochemical detection and HPLC-fluorescence analysis, respectively. Retinol and a-tocopherol were analyzed by using an HPLC system, whereas ascorbate and protein thiols were determined by using spectrophotometry. 8-Hydroxy-2'-deoxyguanosine was visualized by immunofluorescent staining with an anti-8-OHdG antibody that was conjugated with fluorescein isothiocyanate conjugate. Lipid peroxides in spermatozoa were stained with a fluorescent dye, C11-BODIPY581/591.Result(s): Statistically significant negative correlations were revealed between sperm motility and 8-OHdG and between motility and lipid peroxides. Statistically significant positive correlations were found between sperm motility and the levels of retinol, a-tocopherol, ascorbate, and protein thiols of seminal plasma. 8-Hydroxy-2'-deoxyguanosine and lipid peroxides in spermatozoa were found to be present mostly in mitochondria.Conclusion(s): Oxidative stress and oxidative damage were increased significantly in spermatozoa with declined motility, and the antioxidant capacities in the spermatozoa and seminal plasma were lower in males who had infertility or subfertility.