Metatranscriptomics reveals metabolic adaptation and induction of virulence factors by Haemophilus parasuis during lung infection.
Metatranscriptomics reveals metabolic adaptation and induction of virulence factors by Haemophilus parasuis during lung infection.
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DOI：
10.1186/s13567-015-0225-9
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发表时间：
2015-09-23
影响因子：
4.4
通讯作者：
Aragon V
中科院分区：
文献类型：
--
作者：
Bello-Ortí B;Howell KJ;Tucker AW;Maskell DJ;Aragon V
副猪嗜血杆菌（Haemophilus parasuis）是猪上呼吸道的常见寄生菌，也是格拉瑟氏病的病原体。这种疾病的特征是多浆膜炎和关节炎，由细菌的全身传播引起的严重炎症引起。H.在猪感染的早期阶段，猪副伤寒杆菌进入肺部。为了研究该位置的基因表达，我们对离体和体内H.使用元转录组学方法在肺中检测猪副长崎吸虫的转录组。在这些条件下的基因表达与传统的平板培养中发现的比较显示，与合成代谢和分解代谢途径相关的基因的表达普遍减少，再加上与碳获取，铁结合和发病机制有关的膜相关基因的上调。一些上调表达的膜基因，包括ABC转运蛋白、毒力相关的自身转运蛋白（vtaAs）和一些假设的蛋白质，仅在强毒H.副猪链球菌菌株，强调它们作为疾病潜力标志物的重要性。最后，分析还揭示了许多反义转录本的存在，可能在基因调控中发挥作用。总之，这些数据揭示了很少研究的H。parasuis，揭示营养毒力作为宿主生存的适应性策略，除了诱导经典的毒力因子。本文的在线版本（doi：10.1186/s13567-015-0225-9）包含补充材料，可供授权用户使用。
Haemophilus parasuis is a common inhabitant of the upper respiratory tract of pigs, and the causative agent of Glässer’s disease. This disease is characterized by polyserositis and arthritis, produced by the severe inflammation caused by the systemic spread of the bacterium. After an initial colonization of the upper respiratory tract, H. parasuis enters the lung during the early stages of pig infection. In order to study gene expression at this location, we sequenced the ex vivo and in vivo H. parasuis Nagasaki transcriptome in the lung using a metatranscriptomic approach. Comparison of gene expression under these conditions with that found in conventional plate culture showed generally reduced expression of genes associated with anabolic and catabolic pathways, coupled with up-regulation of membrane-related genes involved in carbon acquisition, iron binding and pathogenesis. Some of the up-regulated membrane genes, including ABC transporters, virulence-associated autotransporters (vtaAs) and several hypothetical proteins, were only present in virulent H. parasuis strains, highlighting their significance as markers of disease potential. Finally, the analysis also revealed the presence of numerous antisense transcripts with possible roles in gene regulation. In summary, this data sheds some light on the scarcely studied in vivo transcriptome of H. parasuis, revealing nutritional virulence as an adaptive strategy for host survival, besides induction of classical virulence factors. The online version of this article (doi:10.1186/s13567-015-0225-9) contains supplementary material, which is available to authorized users.