Prediction and functional analysis of native disorder in proteins from the three kingdoms of life
Prediction and functional analysis of native disorder in proteins from the three kingdoms of life
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DOI：
10.1016/j.jmb.2004.02.002
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发表时间：
2004-03-26
影响因子：
5.6
通讯作者：
Jones, DT
中科院分区：
文献类型：
--
作者：
Ward, JJ;Sodhi, JS;Jones, DT
描述了一种从氨基酸序列中识别天然无序区域的自动方法，并对在最新的蛋白质结构预测技术关键评估（CASP）实验中评估的预测因子进行了基准测试。该方法的Wilcoxon评分为90.0，与CASP对相同目标进行评估的方法相比，具有统计学上的显着改善。分类器DISOPRED2被用来估计来自三个生命王国的几个具有代表性的基因组中原生疾病的频率。据推测，在2.0%的古细菌、4.2%的真细菌和33.0%的真核生物中发现了长（bbb30残基）无序片段。利用Saccharomyces基因组数据库提供的基因本体注释，研究了具有长期预测紊乱区域的蛋白质的功能。酵母蛋白质组的分析表明，含有紊乱的蛋白质通常位于细胞核中，并参与转录和细胞信号传导的调节。结果还表明，天然失调与激酶活性和核酸结合的分子功能有关。(C) 2004 Elsevier Ltd.版权所有。
An automatic method for recognizing natively disordered regions from amino acid sequence is described and benchmarked against predictors that were assessed at the latest critical assessment of techniques for protein structure prediction (CASP) experiment. The method attains a Wilcoxon score of 90.0, which represents a statistically significant improvement on the methods evaluated on the same targets at CASP. The classifier, DISOPRED2, was used to estimate the frequency of native disorder in several representative genomes from the three kingdoms of life. Putative, long (>30 residue) disordered segments are found to occur in 2.0% of archaean, 4.2% of eubacterial and 33.0% of eukaryotic proteins. The function of proteins with long predicted regions of disorder was investigated using the gene ontology annotations supplied with the Saccharomyces genome database. The analysis of the yeast proteome suggests that proteins containing disorder are often located in the cell nucleus and are involved in the regulation of transcription and cell signalling. The results also indicate that native disorder is associated with the molecular functions of kinase activity and nucleic acid binding. (C) 2004 Elsevier Ltd. All rights reserved.