NK and CD4 cells collaborate to protect against melanoma tumor formation in the brain
NK and CD4 cells collaborate to protect against melanoma tumor formation in the brain
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DOI：
10.4049/jimmunol.177.12.8448
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发表时间：
2006-12-15
影响因子：
4.4
通讯作者：
Ribas, Antoni
中科院分区：
文献类型：
--
作者：
Prins, Robert M.;Vo, Dan D.;Ribas, Antoni
NK cells represent a potent immune effector cell type that have the ability to recognize and lyse tumors. However, the existence and function of NK cells in the traditionally "immune-privileged" CNS is controversial. Furthermore, the cellular interactions involved in NK cell anti-CNS tumor immunity are even less well understood. We administered non-Ag-loaded, immature dendritic cells (DC) to CD8 alpha knockout (KO) mice and studied their anti-CNS tumor immune responses. DC administration induced dramatic antitumor immune protection in CD8a KO mice that were challenged with B16 melanoma both s.c. and in the brain. The CNS antitumor immunity was dependent on both CD4(+) T cells and NK cells. Administration of non-Ag-loaded, immature DC resulted in significant CD4(+) T cell and NK cell expansion in the draining lymph nodes at 6 days postvaccination, which persisted for 2 wk. Finally, DC administration in CD8a KO mice was associated with robust infiltration of CD4(+) T cells and NK cells into the brain tumor parenchyma. These results represent the first demonstration of a potent innate antitumor immune response against CNS tumors in the absence of toxicity. Thus, non-Ag-loaded, immature DC administration, in the setting of CD8 genetically deficient mice, can induce dramatic antitumor immune responses within the CNS that surpass the effects observed in wild-type mice. Our results suggest that a better understanding of the cross-talk between DC and innate immune cells may provide improved methods to vaccinate patients with tumors located both systemically and within the CNS.
