P-chlorophenylalanine increases glutamate receptor 1 transcription in rat amygdala.
P-chlorophenylalanine increases glutamate receptor 1 transcription in rat amygdala.
复制标题
对氯苯丙氨酸可增加大鼠杏仁核中谷氨酸受体 1 的转录。
DOI：
10.1097/wnr.0b013e32834ae2a1
复制
发表时间：
2011
期刊：
影响因子：
1.7
通讯作者：
Keele,NBradley
中科院分区：
文献类型：
--
作者：
Tran,Lee;Keele,NBradley
杏仁核是一个关键的边缘结构，与癫痫和焦虑症密切相关。癫痫样机制涉及谷氨酸能活动增加，而5-羟色胺[5-羟色胺(5-HT)]系统的紊乱与焦虑样行为有关。以前的研究表明，低5-羟色胺可增加杏仁核的兴奋性，但其分子机制尚不清楚。在这里，我们探讨了低5-羟色胺增加谷氨酸受体转录的能力。利用定量逆转录聚合酶链式反应，我们发现，酪氨酸-5-羟基酶抑制剂P-氯苯丙氨酸处理的大鼠杏仁核中谷氨酸受体1(GluR1)的mRNA表达增加了21倍。这些结果提示，低5-羟色胺通过增加GluR1转录诱导杏仁核神经元兴奋性增强，杏仁核GluR1表达上调可能在焦虑症的病理生理过程中起重要作用。
The amygdala is a key limbic structure strongly implicated in both epilepsy and anxiety disorders. Epilepsy-like mechanisms involve an increased glutamatergic activity, whereas disturbances in serotonin [5-hydroxytryptamine (5-HT)] systems are associated with anxiety-like behavior. Previous studies suggest that low 5-HT increases amygdala excitability, but the molecular mechanisms are not well characterized. Herein we explore the ability of low serotonin to increase glutamate receptor transcription. Using quantitative reverse transcriptase-polymerase chain reaction, we found that rats treated with P-chlorophenylalanine, an inhibitor of tyrosine-5-hydroxylase, resulted in a 21-fold increase in glutamate receptor 1 (GluR1) mRNA expression in the amygdala. These results suggest that low 5-HT induces hyperexcitability of amygdala neurons by increasing GluR1 transcription, and the upregulation of amygdala GluR1 may be important in the pathophysiology of anxiety disorders.