Effect of cyclic mechanical stimulation on the expression of osteogenesis genes in human intraoral mesenchymal stromal and progenitor cells.
Effect of cyclic mechanical stimulation on the expression of osteogenesis genes in human intraoral mesenchymal stromal and progenitor cells.
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DOI：
10.1155/2014/189516
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发表时间：
2014
影响因子：
--
通讯作者：
Leithner A
中科院分区：
文献类型：
--
作者：
Lohberger B;Kaltenegger H;Stuendl N;Payer M;Rinner B;Leithner A
我们评估了机械刺激对人口腔间充质干细胞和祖细胞（MSPCs）成骨分化的影响，使用Flexcell FX 5 K张力系统介导细胞的周期性拉伸。从人下颌磨牙后骨中分离MSPCs，并使用流式细胞术进行表征。CD 73、CD 90和CD 105阳性表达，CD 14、CD 19、CD 34、CD 45和HLA-DR阴性，证实了MSPC表型。平均MSPC倍增时间为30.4 ± 2.1小时。乳酸脱氢酶（LDH）释放的百分比显示机械刺激组和未刺激的对照组之间没有显着差异。逆转录定量实时PCR显示，10%的连续循环应变（0.5 Hz）7和14天诱导成骨分化MSPCs中成骨特异性标志物I型胶原（Col 1A 1）、骨连接蛋白（OPN）、骨形态发生蛋白2（BMP 2）、骨桥蛋白（SPP 1）和骨钙素（BGT）的mRNA表达显著增加。此外，机械刺激组产生的钙沉积到文化和碱性磷酸酶（ALP）的显着更高的金额。这些结果将有助于更好地理解应变诱导的骨重建，并将形成正确选择的作用力在口腔颌面外科手术的基础。
We evaluated the effects of mechanical stimulation on the osteogenic differentiation of human intraoral mesenchymal stem and progenitor cells (MSPCs) using the Flexcell FX5K Tension System that mediated cyclic tensile stretch on the cells. MSPCs were isolated from human mandibular retromolar bones and characterized using flow cytometry. The positive expression of CD73, CD90, and CD105 and negativity for CD14, CD19, CD34, CD45, and HLA-DR confirmed the MSPC phenotype. Mean MSPC doubling time was 30.4 ± 2.1 hrs. The percentage of lactate dehydrogenase (LDH) release showed no significant difference between the mechanically stimulated groups and the unstimulated controls. Reverse transcription quantitative real-time PCR revealed that 10% continuous cyclic strain (0.5 Hz) for 7 and 14 days induced a significant increase in the mRNA expression of the osteogenesis-specific markers type-I collagen (Col1A1), osteonectin (SPARC), bone morphogenetic protein 2 (BMP2), osteopontin (SPP1), and osteocalcin (BGLAP) in osteogenic differentiated MSPCs. Furthermore, mechanically stimulated groups produced significantly higher amounts of calcium deposited into the cultures and alkaline phosphatase (ALP). These results will contribute to a better understanding of strain-induced bone remodelling and will form the basis for the correct choice of applied force in oral and maxillofacial surgery.
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影响因子：
6.2
作者：
Shui, CX;Spelsberg, TC;Khosla, S
通讯作者：
Khosla, S
影响因子：
2.9
作者：
Schallmoser, Katharina;Bartmann, Christina;Strunk, Dirk
通讯作者：
Strunk, Dirk
影响因子：
2.8
作者：
Smith-Adaline, EA;Volkman, SK;Goldstein, SA
通讯作者：
Goldstein, SA
DOI：
10.1073/pnas.202296599
发表时间：
2002-10-01
影响因子：
11.1
作者：
Bancroft, GN;Sikavitsast, VI;Mikos, AG
通讯作者：
Mikos, AG
影响因子：
82.9
作者：
Horwitz, EM;Prockop, DJ;Brenner, MK
通讯作者：
Brenner, MK