CIRCULATING HUMAN MAMMARY EPITHELIAL ANTIGENS IN BREAST-CANCER
CIRCULATING HUMAN MAMMARY EPITHELIAL ANTIGENS IN BREAST-CANCER
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DOI：
10.1073/pnas.79.17.5420
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
BLANK, EW
中科院分区：
文献类型：
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作者：
CERIANI, RL;SASAKI, M;BLANK, EW
针对人乳腺上皮抗原 (HME-Ag) 的异源特异性抗血清存在于人乳脂肪球膜和乳腺上皮细胞中，用于固相放射免疫测定，以确定患有播散性乳腺癌和其他器官的患者血清中这些抗原的存在。乳腺癌患者的循环中携带高水平的 HME-Ag；患有播散性非乳腺癌的患者和正常女性对照则没有。模型系统中显示了循环中类似的 HME-Ag 释放。为了进一步证实这些发现，设计了从血清中提取和鉴定 HME-Ag 的三步程序。在此分析过程中，循环的 HME-Ag 被回收到携带相应抗体（抗 HME）的固相上并进行原位放射性碘化。随后，标记的 HME-Ag 从固相中释放出来，并通过 NaDodSO4 凝胶电泳进行表征。 HME-Ag 是从乳腺癌患者的血清中分离出来的，但不是从非乳腺癌患者或正常女性对照的血清中分离出来的。提取的 HME-Ag 的分子量为 150,000、70,000 和 46,000 道尔顿。针对 46,000 道尔顿 HME-Ag 的单克隆抗体 BLMRL-HMFG-Mc3 也用于从血清中提取其相应的抗原。乳腺癌患者的血清含有这种抗原，而其他患者和对照者的血清则不含这种抗原。这种高度敏感的方法为乳腺癌诊断提供了一种特定的方法，并进一步深入了解循环抗原的性质，以增进对乳腺癌生物学的了解。
Heterologous specific antisera against human mammary epithelial antigens (HME-Ags), which are present in the human milk fat globule membrane and breast epithelial cells, were used in a solid-phase radioimmunoassay to determine the presence of these antigens in the sera of patients with disseminated cancer of the breast and other organs. Breast cancer patients carry high levels of HME-Ags in their circulation; patients with disseminated nonbreast cancer and normal female controls do not. A similar release of HME-Ags in the circulation was shown in a model system. To further corroborate these findings, a 3-step procedure for the extraction and identification of HME-Ags from the sera was devised. In this analytical procedure, circulating HME-Ags are recovered on a solid phase carrying their corresponding antibody (anti-HME) and radioiodinated in situ. Later, the labeled HME-Ags are released from the solid phase and characterized by NaDodSO4 gel electrophoresis. HME-Ags were isolated from sera of breast cancer patients but not from sera of nonbreast cancer patients or of normal female controls. The extracted HME-Ags had molecular masses of 150,000, 70,000 and 46,000 daltons. A monoclonal antibody, BLMRL-HMFG-Mc3, directed to the 46,000-dalton HME-Ag was also used to extract its corresponding antigen from sera. Breast cancer patient sera contained such antigen while the sera of the other patients and controls did not. This highly sensitive methodology offers a specific approach to breast cancer diagnosis and further insight into the nature of circulating antigens to increase understanding of breast cancer biology.