Susceptibility of avian influenza viruses of the N6 subtype to the neuraminidase inhibitor oseltamivir.
Susceptibility of avian influenza viruses of the N6 subtype to the neuraminidase inhibitor oseltamivir.
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DOI：
10.1016/j.antiviral.2011.12.013
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发表时间：
2012-03
影响因子：
7.6
通讯作者：
Webster, Robert G.
中科院分区：
文献类型：
--
作者：
Stoner, Terri D.;Krauss, Scott;Turner, Jasmine C. M.;Seiler, Patrick;Negovetich, Nicholas J.;Stallknecht, David E.;Frase, Sharon;Govorkova, Elena A.;Webster, Robert G.
禽流感病毒是一种遗传物质的来源，可以通过直接引入或重组传播给人类。虽然有大量关于全球监测N1和N2神经氨酸酶（NA）亚型人病毒抗病毒耐药性的信息，但关于这些和其他NA亚型禽流感病毒的信息有限。我们进行了一项监测研究，以调查野生水禽中的主要亚型禽流感N6 NA病毒的抗病毒敏感性。我们评估了73个病毒从北美鸭和滨鸟的敏感性NA抑制剂奥司他韦在基于荧光的NA酶抑制试验。大多数（90%）的平均IC 50值范围为<0.01至5.0 nM; 10%为5.1至50.0 nM;无一>50.0 nM。在大约30年内收集的所有分离株中，对奥司他韦的敏感性保持稳定（P≤0.74）。两个I222 V NA取代的分离株在体外对奥司他韦的敏感性中度降低（IC 50，30.0和40.0 nM）。一个现场样品是一个混合人口含有禽副粘病毒（APMV）和H4 N6流感病毒，显示电子显微镜和血凝抑制试验与一组抗APMV抗血清。这突出了认识和仔细检查禽源现场样本中非流感病原体的重要性。这项研究表明，耐奥司他韦的N6 NA禽流感病毒是罕见的，必须进行测试，表型和基因型，以确认耐药性。
Avian influenza viruses are a source of genetic material that can be transmitted to humans through direct introduction or reassortment. Although there is a wealth of information concerning global monitoring for antiviral resistance among human viruses of the N1 and N2 neuraminidase (NA) subtypes, information concerning avian viruses of these and other NA subtypes is limited. We undertook a surveillance study to investigate the antiviral susceptibility of avian influenza N6 NA viruses, the predominant subtype among wild waterfowl. We evaluated 73 viruses from North American ducks and shorebirds for susceptibility to the NA inhibitor oseltamivir in a fluorescence-based NA enzyme inhibition assay. Most (90%) had mean IC50 values ranging from <0.01 to 5.0 nM; 10% were from 5.1 to 50.0 nM; and none were >50.0 nM. Susceptibility to oseltamivir remained stable among all isolates collected over approximately three decades (P≤0.74). Two isolates with I222V NA substitution had moderately reduced susceptibility to oseltamivir in vitro (IC50, 30.0 and 40.0 nM). One field sample was a mixed population containing an avian paramyxovirus (APMV) and H4N6 influenza virus, as revealed by electron microscopy and hemagglutination inhibition assays with a panel of anti-APMV antisera. This highlights the importance of awareness and careful examination of non-influenza pathogens in field samples from avian sources. This study showed that oseltamivir-resistant N6 NA avian influenza viruses are rare, and must be tested both phenotypically and genotypically to confirm resistance.
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迁徙鸟类中的流感和洲际病毒交换有限的证据。
影响因子：
6.7
作者：
Krauss, Scott;Obert, Caroline A;Franks, John;Walker, David;Jones, Kelly;Seiler, Patrick;Niles, Larry;Pryor, S Paul;Obenauer, John C;Naeve, Clayton W;Widjaja, Linda;Webby, Richard J;Webster, Robert G
通讯作者：
Webster, Robert G
影响因子：
4.9
作者：
Gubareva, LV;Webster, RG;Hayden, FG
通讯作者：
Hayden, FG
影响因子：
4.9
作者：
Govorkova, EA;Leneva, IA;Webster, RG
通讯作者：
Webster, RG
影响因子：
11.8
作者：
Baz, Mariana;Abed, Yacine;Boivin, Guy
通讯作者：
Boivin, Guy
影响因子：
4.9
作者：
Hurt, Aeron C.;Holien, Jessica K.;Barr, Ian G.
通讯作者：
Barr, Ian G.