Orthotopic Xenografts of Colorectal Cancer Stem Cells.
Orthotopic Xenografts of Colorectal Cancer Stem Cells.
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结直肠癌干细胞的原位异种移植。
DOI：
10.1007/978-1-0716-1979-7_39
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发表时间：
2022
影响因子：
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通讯作者：
M. Baiocchi
中科院分区：
文献类型：
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作者：
M. D. De Angelis;Federica Francescangeli;A. Zeuner;M. Baiocchi
癌症干细胞（CSC）负责原发肿瘤的起始和远处器官的转移播种。因此，它们代表了新的抗转移方法的研究和临床前测试的关键目标。我们最近建立了一个结直肠癌干细胞的分子特征生物库，从个体患者中分离出来，并在无血清培养基中培养为多细胞球体。在这里，我们详细描述了基于将 CSC 原位注射到免疫缺陷小鼠盲肠浆膜中的结直肠癌转移模型的生成。这样的模型代表了一个优秀的实验系统，可用于研究结直肠癌转移所涉及的细胞和分子机制，分析罕见的转移前元素（例如循环和播散性肿瘤细胞），以及用于具有潜在抗转移活性的新药物的临床前测试。
Cancer stem cells (CSCs) are responsible for the initiation of primary tumors and for metastasis seeding at distant organs. Therefore, they represent crucial targets for the study and preclinical testing of new antimetastatic approaches. We recently generated a molecularly characterized biobank of colorectal CSCs, isolated from individual patients and cultured in serum-free medium as multicellular spheroids. Here, we describe in detail the generation of a metastatic model of colorectal cancer based on the orthotopic injection of CSCs into the cecum serosa of immunodeficient mice. Such a model represents an excellent experimental system to investigate the cellular and molecular mechanisms involved in colorectal cancer metastasis, to analyze rare premetastatic elements such as circulating and disseminated tumor cells, and for the preclinical testing of new agents with potential antimetastatic activity.