Repair of craniotomy defects using bone marrow stromal cells
Repair of craniotomy defects using bone marrow stromal cells
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DOI：
10.1097/00007890-199811270-00002
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发表时间：
1998-11-27
期刊：
影响因子：
6.2
通讯作者：
Robey, PG
中科院分区：
文献类型：
--
作者：
Krebsbach, PH;Mankani, MH;Robey, PG
背景。用于修复颅面骨缺损的技术与骨骼其他部位骨玻璃的手术治疗方法类似，包括使用自体骨和同种异体材料。移植含有成骨祖细胞的骨髓基质细胞群可能是生成新骨的另一种方式。在免疫功能受损小鼠颅骨中制备全层骨缺损（5mm），并用明胶海绵处理，凝胶海绵中含有携带I型胶原-氯霉素乙酰转移酶报告基因的转基因小鼠的小鼠同种异体骨髓基质细胞，以跟踪移植细胞的命运。对照手术部位单独用脾基质细胞或明胶海绵处理，或不处理。术后2周、3周、4周、6周和12周对手术缺损进行组织学分析。培养的骨髓基质细胞在明胶海绵内移植后成骨，在2周内修复了超过99.0+/-2.20%的原始手术缺损。相比之下，用脾成纤维细胞、单独的载体或假手术对照治疗颅骨缺损，只在手术边缘得到最小程度的修复，携带转基因胶原蛋白的骨髓基质细胞仅在新形成的骨中被免疫检测到，从而证实了移植细胞的供体来源。这些研究表明，有丝分裂扩增的骨髓细胞可以作为骨祖细胞的丰富来源，能够在不添加生长或形态发生因素的情况下修复小鼠颅面骨骼缺陷。
Background. Techniques used to repair craniofacial skeletal defects parallel the accepted surgical therapies for bone lass elsewhere in the skeleton and include the use of autogenous bone and alloplastic materials. Transplantation of a bone marrow stromal cell population that contains osteogenic progenitor cells may be an additional modality for the generation of new bone.Methods. Full thickness osseous defects (5 mm) were prepared in the cranium of immunocompromised mice and were treated with gelatin sponges containing murine alloplastic bone marrow stromal cells derived from transgenic mice carrying a type I collagen-chloramphenicol acetyltransferase reporter gene to follow the fate of the transplanted cells. Control surgical sites were treated with spleen stromal cells or gelatin sponges alone, or were left untreated. The surgical defects were analyzed histologically for percent closure of the defect al 2, 3, 4, 6, and 12 weeks.Results. Cultured bone marrow stromal cells transplanted within gelatin sponges resulted in osteogenesis that repaired greater than 99.0+/-2.20% of the original surgical defect within 2 weeks. In contrast, cranial defects treated with splenic fibroblasts, vehicle alone,, or sham-operated controls resulted in minimal repair that was limited lo the surgical margins, Bone marrow stromal cells carrying the collagen transgene were immunodetected only in the newly formed bone and thus confirmed the donor origin of the transplanted cells.Conclusions. These studies demonstrate that mitotically expanded bone marrow cells can serve as an abundant source of osteoprogenitor cells that are capable of repairing craniofacial skeletal defects in mice without the addition of growth or morphogenetic factors.