A compositional shift in the soil microbiome induced by tetracycline, sulfamonomethoxine and ciprofloxacin entering a plant-soil system
A compositional shift in the soil microbiome induced by tetracycline, sulfamonomethoxine and ciprofloxacin entering a plant-soil system
复制标题
四环素、磺胺间甲氧嘧啶和环丙沙星进入植物-土壤系统引起土壤微生物组的组成变化
DOI：
10.1016/j.envpol.2016.02.043
复制
发表时间：
2016-05-01
影响因子：
8.9
通讯作者：
Ma, Junwei
中科院分区：
文献类型：
--
作者：
Lin, Hui;Jin, Danfeng;Ma, Junwei
进入土壤的抗生素可能会扰乱土壤微生物组复杂的调节网络，而土壤微生物组与土壤质量和生态功能密切相关。研究了四环素（TC）、磺胺间甲氧嘧啶（SMM）、环丙沙星（CIP）及其复配（AM）对土壤-微生物-植物系统中细菌群落的影响，并鉴定了主要的响应菌。抗生素对土壤微生物组的影响取决于抗生素类型和暴露时间。TC导致对土壤微生物组的急性但更迅速地下降的影响，而CIP和SMM导致延迟的抗生素效应。土壤暴露于AM提出了一个高度相似的细菌结构，而不是暴露于TC SMM和CIP。TC、SMM和CIP具有其自身的主要受影响的分类群，包括耐药性和敏感性细菌。抗生素敏感反应者主要分布在变形菌门。对每种抗生素的潜在耐药菌均表现出一定程度的门偏好性，尤其是TC耐药菌。随着暴露时间的延长，土壤对CIP和SMM的抗性增加，而对TC的抗性则相反。总的来说，这项工作扩展了对抗生素在温室蔬菜种植期间引入土壤后对土壤微生物组影响的理解。(C)2016爱思唯尔有限公司版权所有
Antibiotics entering the soil likely disturb the complex regulatory network of the soil microbiome, which is closely associated with soil quality and ecological function. This study investigated the effects of tetracycline (TC), sulfamonomethoxine (SMM), ciprofloxacin (CIP) and their combination (AM) on the bacterial community in a soil-microbe-plant system and identified the main bacterial responders. Antibiotic effects on the soil microbiome depended on antibiotic type and exposure time. TC resulted in an acute but more rapidly declining effect on soil microbiome while CIP and SMM led to a delayed antibiotic effect. The soil exposed to AM presented a highly similar bacterial structure to that exposed to TC rather than to SMM and CIP. TC, SMM and CIP had their own predominantly impacted taxonomic groups that include both resistance and sensitive bacteria. The antibiotic sensitive responders predominantly distributed within the phylum Proteobacteria. The potential bacteria resistant to each antibiotic exhibited phyla preference to some extent, particularly those resistant to TC. CIP and SMM resistance in soil was increased with exposure time while TC resistance gave the opposite result. Overall, the work extended the understanding of antibiotic effects on soil microbiome after introduced into the soil during greenhouse vegetable cultivation. (C) 2016 Elsevier Ltd. All rights reserved.