HIGH-AFFINITY ANTIBODY FROM HENS EGGS DIRECTED AGAINST THE HUMAN INSULIN-RECEPTOR AND THE HUMAN IGF-I RECEPTOR
HIGH-AFFINITY ANTIBODY FROM HENS EGGS DIRECTED AGAINST THE HUMAN INSULIN-RECEPTOR AND THE HUMAN IGF-I RECEPTOR
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DOI：
10.1016/0003-2697(88)90171-6
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发表时间：
1988-08-15
影响因子：
2.9
通讯作者：
GREEN, A
中科院分区：
文献类型：
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作者：
STUART, CA;PIETRZYK, RA;GREEN, A
从鸡卵中获得了大量抗人胰岛素受体和人胰岛素样生长因子-I（IGF-I）受体的高亲和力抗体。用人胎盘膜和人肝膜肌肉注射免疫母鸡。5周时血清和蛋黄中均出现了特异性抗体，这些抗体至少可持续6个月。用Polson等人的聚乙二醇沉淀技术纯化来自蛋黄的抗体。鸡蛋提供相当于每只鸡每月约450 ml免疫血清的量。纯化的抗体与胰岛素或IGF-I受体的反应性大致相等。抗体免疫沉淀亲和标记的受体，抑制每个配体的结合，并能够刺激2-脱氧葡萄糖摄取大鼠脂肪细胞和胸苷掺入培养的成纤维细胞。在用人肝膜免疫的那些母鸡中存在针对IGF-I受体的抗体是出乎意料的，因为肝膜具有很少或没有IGF-I结合活性。我们的结论是，通过免疫母鸡和从鸡蛋中纯化这些抗体，可以相对容易地获得抗人抗原的抗体。
Large quantities of high affinity antibodies directed against the human insulin receptor and the human insulin-like growth factor-I (IGF-I) receptor were obtained from hen''s eggs. Hens were immunized with human placental membranes and human liver membranes by intramuscular injections. Specific antibodies to the receptors were demonstrated in serum and egg yolks at 5 weeks and these antibodies presisted for at least 6 months. Antibodies from egg yolks were purified by the polyethylene glycol precipitation technique of Polson et al. The eggs provided the equivalent of about 450 ml of immunized serum per month per bird. The purified antibodies were approximately equally reactive with receptors for insulin or IGF-I. Antibodies immunoprecipitated affinity-labeled receptors, inhibited binding of each ligand, and were capable of stimulating 2-deoxyglucose uptake in rat adipocytes and thymidine incorporation in cultured fibroblasts. The presence of antibodies directed against the IGF-I receptor in those hens immunized with human liver membranes was unexpected, since liver membranes possess little or no IGF-I binding activity. We concluded that antibodies against human antigens may be relatively easily obtainedc by immunization of hens and purfication of those antibodies from eggs.