Biotransformations of carboxylated aromatic compounds by the acetogen Clostridium thermoaceticum: generation of growth-supportive CO2 equivalents under CO2-limited conditions.
Biotransformations of carboxylated aromatic compounds by the acetogen Clostridium thermoaceticum: generation of growth-supportive CO2 equivalents under CO2-limited conditions.
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产乙酸菌热乙酸梭菌对羧化芳香族化合物的生物转化：在 CO2 限制条件下产生支持生长的 CO2 当量。
DOI：
10.1128/jb.172.1.212-217.1990
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发表时间：
1990
影响因子：
3.2
通讯作者：
Drake,HL
中科院分区：
文献类型：
--
作者：
Hsu,T;Daniel,SL;Lux,MF;Drake,HL
热醋酸梭菌ATCC 39073将香草酸转化为儿茶酚。虽然不含甲氧基的羧基芳香族化合物本身不支持生长，但原儿茶酸盐和对羟基苯甲酸盐（非甲氧基芳香族化合物）在依赖一氧化碳的生长过程中分别转化为儿茶酚和苯酚。Syringate不受C. thermoaceticum脱羧的影响（Z. Wu， S. L. Daniel, and H. L. Drake， J. Bacteriol. 170:5705- 5708,1988），以牺牲Syringate衍生的甲氧基为代价的持续生长依赖于补充CO2。相比之下，在没有添加二氧化碳的情况下，香草素有利于生长，而在[羧基- 14c]香草素依赖性生长过程中，14CO2是主要的14c标记产物。此外，当生长培养基中补充的二氧化碳耗尽时，原儿茶酸酯和对羟基苯甲酸酯的脱羧支持甲醇和1,2,3-三甲氧基苯依赖性生长（以牺牲这些底物为代价生长需要二氧化碳），并且原儿茶酸酯的脱羧伴随着甲醇培养细胞产量的提高。这些发现表明：(1)C. thermoaceticum能够对某些芳香族化合物进行脱羧反应；(2)在一定条件下，脱羧反应可能与丙酮生成过程中的碳和能量流动相结合。
Clostridium thermoaceticum ATCC 39073 converted vanillate to catechol. Although carboxylated aromatic compounds which did not contain methoxyl groups were not by themselves growth supportive, protocatechuate and p-hydroxybenzoate (nonmethoxylated aromatic compounds) were converted to catechol and phenol, respectively, during carbon monoxide-dependent growth. Syringate is not subject to decarboxylation by C. thermoaceticum (Z. Wu, S. L. Daniel, and H. L. Drake, J. Bacteriol. 170:5705-5708, 1988), and sustained growth at the expense of syringate-derived methoxyl groups was dependent on supplemental CO2. In contrast, vanillate was growth supportive in the absence of supplemental CO2, and 14CO2 was the major 14C-labeled product during [carboxyl-14C]vanillate-dependent growth. Furthermore, the decarboxylation of protocatechuate and p-hydroxybenzoate supported methanol- and 1,2,3-trimethoxybenzene-dependent growth (CO2 is required for growth at the expense of these substrates) when supplemental CO2 was depleted from the growth medium, and the decarboxylation of protocatechuate was concomitant with improved cell yields of methanol cultures. These findings demonstrate that (i) C. thermoaceticum is competent in the decarboxylation of certain aromatic compounds and (ii) under certain conditions, decarboxylation may be integrated to the flow of carbon and energy during acetogenesis.