Atomic force microscopy for characterization of the biomaterial interface
Atomic force microscopy for characterization of the biomaterial interface
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DOI：
10.1016/s0142-9612(97)00222-6
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发表时间：
1998-03-01
期刊：
影响因子：
14
通讯作者：
Marchant, RE
中科院分区：
文献类型：
--
作者：
Siedlecki, CA;Marchant, RE
在植入生物材料的界面处发生的分子过程决定了宿主的反应，包括蛋白质吸附、构象变化和随后与细胞组分的相互作用等现象。直到最近，这些过程还不能直接观察到。在过去的十年中，原子力显微镜（AFM）提供了在生物材料界面发生的分子水平的相互作用的机械见解。已经开发了几种独特的操作模式，其利用与样品的间歇接触并减少施加的剪切力。这些动力学模型也可用于研究不同结构组分对生物材料细观力学性能的影响。力检测技术允许个体受体-配体结合事件的分子水平研究，以及用于确定结构/功能关系的力映射。尖端制造、图像处理技术、模型表面的使用和标记的进步都有助于AFM作为最先进的研究仪器的进步。在这份报告中，我们研究的生物材料和细胞/分子相互作用的研究的AFM的适用性。(C)1998由Elsevier Science Ltd.出版。保留所有权利。
The molecular processes that occur at the interface of an implanted biomaterial determines the host response, including phenomena such as protein adsorption, conformational changes and subsequent interactions with cellular components. Until recently, such processes could not be observed directly. Over the past decade, atomic force microscopy (AFM) has provided mechanistic insights into the molecular level interactions that occur at the biomaterial interface. Several unique operational modes have been developed which utilize intermittent contact with the sample and decrease applied shear forces. These dynamic modes also can be used to study the role of different structural components on biomaterial micromechanical properties. Force detection techniques allow molecular level studies of individual receptor-ligand binding events, and force mapping for determining structure/function relationships. Advancements in tip manufacturing, image processing techniques, the use of model surfaces and labeling all have contributed to the advancement of the AFM as a state-of-the-art research instrument. In this report, we examine the applicability of the AFM to the study of biomaterials and cell/molecular interactions. (C) 1998 Published by Elsevier Science Ltd. All rights reserved.