Lysionotin attenuates Staphylococcus aureus pathogenicity by inhibiting alpha-toxin expression
Lysionotin attenuates Staphylococcus aureus pathogenicity by inhibiting alpha-toxin expression
复制标题
Lysionotin 通过抑制 α 毒素表达来减弱金黄色葡萄球菌的致病性
DOI：
10.1007/s00253-017-8417-z
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发表时间：
2017
影响因子：
5
通讯作者：
Wang Jianfeng
中科院分区：
文献类型：
--
作者：
Teng Zihao;Shi Dongxue;Liu Huanyu;Shen Ziying;Zha Yonghong;Li Wenhua;Deng Xuming;Wang Jianfeng
α-Toxin, one of the best known pore-forming proteins produced byStaphylococcus aureus(S. aureus), is a critical virulence factor in multiple infections. The necessity of α-toxin forS. aureuspathogenicity suggests that this toxin is an important target for the development of a potential treatment strategy. In this study, we showed that lysionotin, a natural compound, can inhibit the hemolytic activity of culture supernatants byS. aureusby reducing α-toxin expression. Using real-time PCR analysis, we showed that transcription ofhla(the gene encoding α-toxin) andagr(the locus regulatinghla) was significantly inhibited by lysionotin. Lactate dehydrogenase and live/dead assays indicated that lysionotin effectively protected human alveolar epithelial cells againstS. aureus, and in vivo studies also demonstrated that lysionotin can protect mice from pneumonia caused byS. aureus. These findings suggest that lysionotin is an efficient inhibitor of α-toxin expression and shows significant protection againstS. aureusin vitro and in vivo. This study supports a potential strategy for the treatment ofS. aureusinfection by inhibiting the expression of virulence factors and indicates that lysionotin may be a potential treatment forS. aureuspneumonia.
