High-molecular weight kininogen is present in cultured human endothelial cells: localization, isolation, and characterization.
High-molecular weight kininogen is present in cultured human endothelial cells: localization, isolation, and characterization.
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高分子量激肽原存在于培养的人内皮细胞中：定位、分离和表征。
作者：
F. van Iwaarden;P. D. de Groot;J. Sixma;M. Berrettini;B. Bouma
高分子量（摩尔重量）激肽原的存在下，证明在培养的人内皮细胞来自脐带的免疫荧光技术。培养的人内皮细胞含有58 +/- 11 ng（n = 16）高分子量激肽原/10（6）个细胞，这是通过对高分子量激肽原特异性的酶联免疫吸附测定（ELISA）确定的。用免疫亲和层析法从培养的人内皮细胞中分离得到高分子量激肽原。非还原性十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）显示内皮细胞高分子量激肽原由5条蛋白带组成，分子量分别为95，000、85，000、65，000、46，000和30，000道尔顿。使用抗重链和轻链的特异性抗血清对内皮细胞高分子量激肽原进行免疫印迹，结果表明95，000-、85，000-和65，000-道尔顿条带由重链和轻链组成，而46，000-和30，000-道尔顿条带仅与抗轻链抗血清反应。用裂解的代谢标记的内皮细胞和针对高分子量激肽原的单特异性抗血清进行的免疫沉淀研究表明，高分子量激肽原不由内皮细胞合成。在高分子量激肽原培养基中培养的内皮细胞不含高分子量激肽原。这些研究表明，内皮细胞高分子量激肽原在培养基中被蛋白水解裂解，随后被内皮细胞内化。结合和内化与125碘标记，蛋白水解裂解，高分子量激肽原的研究表明，内皮细胞确实可以结合和内化蛋白水解裂解高分子量激肽原在一个特定的和饱和的方式。
The presence of high-molecular weight (mol wt) kininogen was demonstrated in cultured human endothelial cells derived from the umbilical cord by immunofluorescence techniques. Cultured human endothelial cells contain 58 +/- 11 ng (n = 16) high-mol wt kininogen/10(6) cells as determined by an enzyme-linked immunosorbent assay (ELISA) specific for high-mol wt kininogen. High-mol wt kininogen was isolated from cultured human endothelial cells by immunoaffinity chromatography. Nonreduced sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that endothelial cell high-mol wt kininogen consisted of five protein bands with mol wts of 95,000, 85,000, 65,000, 46,000, and 30,000 daltons. Immunoblotting of the endothelial cell high-mol wt kininogen by using specific antisera against the heavy and light chain indicated that the 95,000-, 85,000-, and 65,000-dalton bands consisted of the heavy and light chain whereas the 46,000- and 30,000-dalton bands reacted only with the anti-light chain antiserum. Immunoprecipitation studies performed with lysed, metabolically labeled endothelial cells and monospecific antisera directed against high-mol wt kininogen suggested that high-mol wt kininogen is not synthesized by the endothelial cells. Endothelial cells cultured in high-mol wt kininogen-free medium did not contain high-mol wt kininogen. These studies indicate that endothelial cell high-mol wt kininogen was proteolytically cleaved in the culture medium and subsequently internalized by the endothelial cells. Binding and internalization studies performed with 125I-labeled, proteolytically cleaved, high-mol wt kininogen showed that endothelial cells can indeed bind and internalize proteolytically cleaved high-mol wt kininogen in a specific and saturable way.
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通过单克隆抗体免疫印迹检测人血浆中高分子量激肽原的体外和体内裂解。
影响因子：
20.3
作者：
Berrettini,M;Lämmle,B;White,T;Heeb,MJ;Schwarz,HP;Zuraw,B;Curd,J;Griffin,JH
通讯作者：
Griffin,JH
对正常血浆和缺陷血浆以及冷依赖性激活后的正常血浆中的前激肽释放酶、激肽释放酶和高分子量激肽原进行免疫学研究。
DOI：
--
发表时间：
1980
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Bouma,BN;Kerbiriou,DM;Vlooswijk,RA;Griffin,JH
通讯作者：
Griffin,JH
DOI：
10.1073/pnas.82.8.2523
发表时间：
1985
影响因子：
11.1
作者：
Stern,D;Nawroth,P;Handley,D;Kisiel,W
通讯作者：
Kisiel,W
抗凝血酶 III 与牛主动脉段的相互作用。
DOI：
10.1172/jci111685
发表时间：
1985
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Stern,D;Nawroth,P;Marcum,J;Handley,D;Kisiel,W;Rosenberg,R;Stern,K
通讯作者：
Stern,K
与培养的牛主动脉内皮细胞结合的因子 IX 的激活。
DOI：
10.1073/pnas.81.3.913
发表时间：
1984
影响因子：
11.1
作者：
Stern,DM;Drillings,M;Kisiel,W;Nawroth,P;Nossel,HL;LaGamma,KS
通讯作者：
LaGamma,KS