Nanoengineering Hybrid Supramolecular Multilayered Biomaterials Using Polysaccharides and Self-Assembling Peptide Amphiphiles
Nanoengineering Hybrid Supramolecular Multilayered Biomaterials Using Polysaccharides and Self-Assembling Peptide Amphiphiles
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DOI：
10.1002/adfm.201605122
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发表时间：
2017-05-04
影响因子：
19
通讯作者：
Mano, Joao F.
中科院分区：
文献类型：
--
作者：
Borges, Joao;Sousa, Maria P.;Mano, Joao F.
通过高度动态和可逆的非共价相互作用开发复杂的超分子生物材料引起了科学界的高度关注，其目标是关键的生物医学和生物技术应用，包括组织工程、再生医学或药物输送。在这项研究中，作者报告了通过自组装和静电驱动的逐层（LbL）组装方法相结合，制造了混合超分子多层生物材料，包括高分子量生物聚合物和带相反电荷的低分子量肽两亲物（PA）。海藻酸盐是一种阴离子多糖，用于触发带正电荷的 PA 的自组装能力和一维纳米纤维网络的形成。 LbL技术进一步用于通过重复两种分子的交替沉积来制造超分子多层生物材料。制造过程由石英晶体微天平监测，表明两种材料可以成功组合以构想稳定的超分子系统。通过先进的显微镜技术研究了系统的形态特性，揭示了两个分子形成的组装体的纳米结构尺寸和一维纳米纤维网络。在以 PA 作为最外层的纳米结构上观察到增强的 C2C12 细胞粘附、增殖和分化。这种超分子生物材料被证明是细胞培养的创新基质，并且在不久的将来作为有前景的仿生超分子纳米平台在实际应用中具有巨大的潜力。
Developing complex supramolecular biomaterials through highly dynamic and reversible noncovalent interactions has attracted great attention from the scientific community aiming key biomedical and biotechnological applications, including tissue engineering, regenerative medicine, or drug delivery. In this study, the authors report the fabrication of hybrid supramolecular multilayered biomaterials, comprising high-molecular-weight biopolymers and oppositely charged low-molecular-weight peptide amphiphiles (PAs), through combination of self-assembly and electrostatically driven layer-by-layer (LbL) assembly approach. Alginate, an anionic polysaccharide, is used to trigger the self-assembling capability of positively charged PA and formation of 1D nanofiber networks. The LbL technology is further used to fabricate supramolecular multilayered biomaterials by repeating the alternate deposition of both molecules. The fabrication process is monitored by quartz crystal microbalance, revealing that both materials can be successfully combined to conceive stable supramolecular systems. The morphological properties of the systems are studied by advanced microscopy techniques, revealing the nanostructured dimensions and 1D nanofibrous network of the assembly formed by the two molecules. Enhanced C2C12 cell adhesion, proliferation, and differentiation are observed on nanostructures having PA as outermost layer. Such supramolecular biomaterials demonstrate to be innovative matrices for cell culture and hold great potential to be used in the near future as promising biomimetic supramolecular nanoplatforms for practical applications.