DNA sequence of the lactose operon: the lacA gene and the transcriptional termination region.
DNA sequence of the lactose operon: the lacA gene and the transcriptional termination region.
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乳糖操纵子的 DNA 序列：lacA 基因和转录终止区。
DOI：
10.1073/pnas.82.19.6414
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发表时间：
1985
影响因子：
11.1
通讯作者：
Zabin,I
中科院分区：
文献类型：
--
作者：
Hediger,MA;Johnson,DF;Nierlich,DP;Zabin,I
大肠杆菌的lac操纵子跨越约5300个碱基对，并且除了操纵子、启动子和转录终止区之外还包括lacZ、lacY和lacA基因。我们在这里报告的lacA基因的序列和该地区的远端，确认硫代半乳糖苷转乙酰酶的序列，并完成了乳糖操纵子的序列。lacA基因的特征在于使用稀有密码子，表明其来源于质粒、转座子或病毒基因。UUG是翻译起始密码子。对lacA基因远端区域中的lac信使的3'端的初步检查表明了几个终点。主要的一个位于富含G + C的发夹结构的3'端，其可能参与转录终止或转录后加工。lacA下游的互补链上存在702个碱基对的开放阅读框。
The lac operon of Escherichia coli spans approximately 5300 base pairs and includes the lacZ, lacY, and lacA genes in addition to the operator, promoter, and transcription termination regions. We report here the sequence of the lacA gene and the region distal to it, confirming the sequence of thiogalactoside transacetylase and completing the sequence of the lac operon. The lacA gene is characterized by use of rare codons, suggesting an origin from a plasmid, transposon, or virus gene. UUG is the translation initiation codon. A preliminary examination of 3' end of the lac messenger in the region distal to the lacA gene indicates several endpoints. A predominant one is located at the 3' end of a G + C-rich hairpin structure, which may be involved in termination of transcription or in post-transcriptional processing. An open reading frame of 702 base pairs is present on the complementary strand downstream from lacA.