Evaluation of degenerative changes in articular cartilage of osteoarthritis by Raman spectroscopy
Evaluation of degenerative changes in articular cartilage of osteoarthritis by Raman spectroscopy
复制标题
拉曼光谱评价骨关节炎关节软骨退行性改变
DOI：
10.1117/12.2291383
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Hino Kazunori
中科院分区：
文献类型：
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作者：
Oshima Yusuke;Kiyomatsu Hiroshi;Miura Hiromasa;Ishimaru Yasumitsu;Hino Kazunori
骨关节炎（OA）是老年人群中非常常见的关节疾病。OA的主要症状是伴有关节软骨的退行性改变。软骨主要含有II型胶原和蛋白多糖，因此难以通过常规诊断工具（X射线、MRI和回声描记术）直接或间接地获得软骨组织的质量和形态。拉曼光谱是一种无标记技术，能够分析退行性软骨中的分子组成。本研究的目的是发展一套拉曼光谱系统，用于关节镜手术中关节软骨的质量评估。为了实现这一目标，我们专注于软骨基质中的蛋白多糖含量和胶原纤维排列，这可能与OA的退行性变化有关，我们设计了一种原始的拉曼装置，用于关节镜手术期间的遥感。在这个项目中，我们定义了基于拉曼光谱的软骨缺损分级系统，我们完成了评估的拉曼探测系统，这使得有可能检测到早期退行性软骨作为一种新的工具，用于OA诊断使用人类受试者。
Osteoarthritis (OA) is a very common joint disease in the aging population. Main symptom of OA is accompanied by degenerative changes of articular cartilage. Cartilage contains mostly type II collagen and proteoglycans, so it is difficult to access the quality and morphology of cartilage tissue in situ by conventional diagnostic tools (X-ray, MRI and echography) directly or indirectly. Raman spectroscopy is a label-free technique which enables to analyze molecular composition in degenerative cartilage. In this proposal, we aim to develop Raman spectroscopic system for the quality assessment of articular cartilage during arthroscopic surgery. Toward this goal, we are focusing on the proteoglycan content and collagen fiber alignment in cartilage matrix which may be associated with degenerative changes in OA, and we designed an original Raman device for remote sensing during arthroscopic surgery. In this project, we define the grading system for cartilage defect based on Raman spectroscopy, and we complete the evaluation of the Raman probing system which makes it possible to detect early stage of degenerative cartilage as a novel tool for OA diagnosis using human subject.