Yeast Dam1p is required to maintain spindle integrity during mitosis and interacts with the Mps1p kinase
Yeast Dam1p is required to maintain spindle integrity during mitosis and interacts with the Mps1p kinase
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DOI：
10.1091/mbc.10.7.2377
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发表时间：
1999-07-01
影响因子：
3.3
通讯作者：
Winey, M
中科院分区：
文献类型：
--
作者：
Jones, MH;Bachant, JB;Winey, M
我们在筛选突变时发现了 DAM1 基因的突变等位基因，该突变与 mps1-1 突变结合致命。 MPS1 编码一种必需的蛋白激酶，是纺锤体极体复制和纺锤体组装检查点所必需的。六个不同基因的突变被发现与 mps1-1 结合是致命的，其中只有 DAM1 是新的。其余基因编码检查点蛋白 Bub1p 和四种伴侣蛋白 Sti1p、Hsc82p、Cdc37p 和 Ydj1p。 DAM1 是一种必需基因，编码最近被描述为微管结合复合物成员的蛋白质。我们在此报告，携带 dam1-1 突变的细胞在限制温度下的后期无法维持纺锤体的完整性。与这种表型一致，DAM1 显示与 STU1、CIN8 和 KAR3（编码参与纺锤体功能的蛋白质的基因）的遗传相互作用。我们观察到，在内源水平表达并通过免疫荧光显微镜定位的 Dam1p-Myc 融合蛋白似乎沿着短有丝分裂纺锤体均匀分布，但在有丝分裂后期发现于纺锤体极处。
We have identified a mutant allele of the DAM1 gene in a screen for mutations that are lethal in combination with the mps1-1 mutation. MPS1 encodes an essential protein kinase that is required for duplication of the spindle pole body and for the spindle assembly checkpoint. Mutations in six different genes were found to be lethal in combination with mps1-1, of which only DAM1 was novel. The remaining genes encode a checkpoint protein, Bub1p, and four chaperone proteins, Sti1p, Hsc82p, Cdc37p, and Ydj1p. DAM1 is an essential gene that encodes a protein recently described as a member of a microtubule binding complex. We report here that cells harboring the dam1-1 mutation fail to maintain spindle integrity during anaphase at the restrictive temperature. Consistent with this phenotype, DAM1 displays genetic interactions with STU1, CIN8, and KAR3, genes encoding proteins involved in spindle function. We have observed that a Dam1p-Myc fusion protein expressed at endogenous levels and localized by immunofluorescence microscopy, appears to be evenly distributed along short mitotic spindles but is found at the spindle poles at later times in mitosis.