Changes in the ovarian dynamics and endocrine profiles in goats treated with a progesterone antagonist during the early luteal phase of the estrous cycle
Changes in the ovarian dynamics and endocrine profiles in goats treated with a progesterone antagonist during the early luteal phase of the estrous cycle
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DOI：
10.1016/j.anireprosci.2006.09.015
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发表时间：
2007-10-01
影响因子：
2.2
通讯作者：
Kamornae, Hideo
中科院分区：
文献类型：
--
作者：
Suganuma, Chiho;Kuroiwa, Takenobu;Kamornae, Hideo
本研究的目的是确定内源性黄体酮在调节山羊黄体期早期卵巢动态、促性腺激素和黄体酮分泌中的生理作用。通过经直肠超声检查确定排卵后 1 至 7 天（第 0 天），循环柴山羊每天皮下注射媒介物（对照组，n = 5）或 50 mg RU486（RU486 组，n = 4）。通过超声检查监测卵巢动态，并每天采集血样直至随后排卵，用于分析黄体酮、黄体生成素（LH）和卵泡刺激素（FSH）的分泌。在第 3 天和第 7 天的 6 小时内，每隔 10 分钟采集一次血样，用于分析 LH 和 FSH 的脉动模式。第 7 天，RU486 组的 LH 脉冲频率显着高于对照组（4.8+/-1.1 脉冲/6 小时 vs 1.2+/-0.4 脉冲/6 小时）（P < 0.05）。两组中第 3 天和第 7 天的 FSH 脉冲形状均不清楚，第 3 天和第 7 天 6 小时的 FSH 浓度总体平均值在 RU486 组和对照组之间没有显着差异。两组的每日 FSH 浓度模式均呈现波浪状波动。 RU486 组和对照组之间每日 FSH 分泌的波状模式的峰间间隔没有显着差异（4.1+/-0.6 天与 4.5+/-0.6 天）。 RU486 组从第 1 天到第 7 天生长的最大卵泡的最大直径往往大于对照山羊（6.4+/-0.8 mm 与 5.0+/-0.8 mm，P = 0.050），而在治疗期间的几乎所有天，对照组和 RU486 组之间的黄体大小和孕酮浓度没有检测到显着差异。这些结果表明，孕酮浓度的升高抑制了山羊动情周期早期黄体期的搏动性LH分泌和卵泡生长，而孕酮在调节FSH分泌和黄体功能中没有生理作用。 (c) 2001 Elsevier B.V. 保留所有权利。
The aim of the present study was to determine the physiological role of endogenous progesterone in the regulation of ovarian dynamics, gonadotropin and progesterone secretion during the early luteal phase in the goat. Cycling Shiba goats received subcutaneously a vehicle (control group, n = 5) or 50 mg of RU486 (RU486 group, n = 4) daily from I to 7 days after ovulation (day 0) determined by transrectal ultrasonography. Ovarian dynamics were monitored by the ultrasonography and blood samples were collected daily until the subsequent ovulation for analysis of progesterone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) secretion. Blood samples were also collected at 10 min intervals for 6 h on day 3 and day 7 for the analysis of pulsatile patterns of LH and FSH. The LH pulse frequency was significantly (P < 0.05) higher in the RU486 group than in the control group on day 7 (4.8+/-1.1 pulses/6 h versus 1.2+/-0.4 pulses/6 h). The shape of the FSH pulses was unclear on day 3 and day 7 in both groups and the overall means of FSH concentration for 6 h on day 3 and day 7 were not significantly different between the RU486 and the control groups. The pattern of daily FSH concentrations showed a wave-like fluctuation in both groups. There was no significant difference in the inter-peak intervals of the wave-like pattern of daily FSH secretion between the RU486 and the control groups (4.1+/-0.6 days versus 4.5+/-0.6 days). The maximum diameter of the largest follicle that grew from day 1 to day 7 in the RU486 group tended to be greater than that in control goats (6.4+/-0.8 mm versus 5.0+/-0.8 mm, P = 0.050), whereas no significant difference was detected in the size of the corpus luteum and progesterone concentrations between the control and RU486 groups on almost all days during the treatment period. These results indicate that the rise of the progesterone concentration suppresses the pulsatile LH secretion and follicular growth, whereas progesterone has no physiological role in the regulation of FSH secretion and luteal function during the early luteal phase of the estrous cycle in goats. (c) 2001 Elsevier B.V. All rights reserved.