A label-free DNAzyme fluorescence biosensor for amplified detection of Pb2+-based on cleavage-induced G-quadruplex formation
A label-free DNAzyme fluorescence biosensor for amplified detection of Pb2+-based on cleavage-induced G-quadruplex formation
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DOI：
10.1016/j.talanta.2015.10.004
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发表时间：
2016-01-15
期刊：
影响因子：
6.1
通讯作者：
Tan, Weihong
中科院分区：
文献类型：
--
作者：
Fu, Ting;Ren, Songlei;Tan, Weihong
基于DNA酶的催化信标已被广泛研究用于体内和体外分子检测。然而，迄今为止，只有少数具有优异分析性能的无标记催化信标被报道。在这项工作中，通过结合催化DNAzyme的放大传感通过酶的营业额与裂解诱导的G-四链体的形成，无标记的DNAzyme生物传感器的开发用于放大的“打开”荧光检测的Pb 2+的检测限为3 nM。该方法是非常有竞争力的许多其他标记或无标记的方法相比，有或没有信号放大。由于GR-5 DNAzyme的固有特异性，该方法也表现出优异的选择性。该生物传感器成功地检测了河水样品中的Pb 2+，具有较高的灵敏度和选择性。这种方法可能提供一个通用的DNA酶为基础的传感平台，灵敏地检测各种目标在环境和生物医学领域。(C)2015 Elsevier B. V.版权所有。
DNAzyme-based catalytic beacons have been widely studied for both in vivo and in vitro molecular detection. However, only a few label-free catalytic beacons with excellent analytical performance have been reported so far. In this work, by combining a catalytic DNAzyme for amplified sensing through enzymatic turnover with cleavage-induced G-quadruplex formation, a label-free DNAzyme biosensor was developed for amplified "turn-on" fluorescence detection of Pb2+ with a detection limit of 3 nM. The method is very competitive compared to many other labeled or label-free methods with or without signal amplification. Due to the inherent specificity of the GR-5 DNAzyme, the method also exhibits excellent selectivity. This biosensor successfully detected Pb2+ in river water samples with high sensitivity and selectivity. Such a method might provide a universal DNAzyme-based sensing platform for sensitive detection of various targets both in environmental and biomedical fields. (C) 2015 Elsevier B.V. All rights reserved.