Production and purification of agarase from a marine agarolytic bacterium Agarivorans sp HZ105
Production and purification of agarase from a marine agarolytic bacterium Agarivorans sp HZ105
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DOI：
10.1111/j.1365-2672.2008.03990.x
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发表时间：
2009-01-01
影响因子：
4
通讯作者：
Liu, G. -M.
中科院分区：
文献类型：
--
作者：
Hu, Z.;Lin, B. -K.;Liu, G. -M.
琼胶酶产生菌的分离及酶学性质研究从海洋沉积物中分离到一株琼胶酶产生菌HZ 105。根据16 S rRNA基因序列的系统发育分析、表型分析和生化分析，将该菌株命名为Agarivorans sp. HZ 105。研究了pH、NaCl对菌株HZ 105生长和琼胶酶产生的影响。菌株HZ 105产生三种胞外琼脂糖酶，其从PAGE凝胶中的条带纯化至均一。这三种琼胶酶中有两种的分子量分别为54，58 kDa。利用MS和MS/MS谱对琼胶酶进行了鉴定，结果表明菌株HZ 105产生的琼胶酶属于糖基水解酶家族50的β-琼胶酶。该酶在室温下具有稳定的酶活，表明菌株HZ 105具有良好的产酶能力。琼脂在琼脂平板中降解产生的溶液首次被报道用于琼脂酶的纯化。直接从PAGE凝胶中纯化琼脂糖酶至均一，而不经考马斯亮蓝染色。
Isolation and characterization of an agarase-producing bacterium Agarivorans sp. HZ105.An agarase-producing bacterium strain HZ105 had been isolated from marine sediment sample. Based on phylogenetic analysis of the 16S rRNA gene sequence and phenotypic analysis, as well as biochemical analyses, this strain was named Agarivorans sp. HZ105. Effect of pH, NaCl on the growth and agarase production of strain HZ105 was studied. Strain HZ105 produced three extracellular agarases which were purified to homogeneity from bands in the PAGE gel. Two agarases of these three had a molecular mass of 54, 58 kDa, respectively. And the MS and MS/MS spectra were used to identify the agarases.The MS spectra result showed that the agarases of strain HZ105 should be beta-agarase and belong to the family 50 of glycosyl hydrolases. The agarases could keep stable activity at room temperature.The strain HZ105 was useful to produce stable agarases. The solution produced by agar's degradation in the agar plates was first reported to be used for purification of agarase. Agarases were purified to homogeneity directly from the PAGE gel without stained by Coomassie brilliant blue.