The effect of light quality on the morphogenesis of in vitro cultures of Azorina vidalii (Wats.) Feer
The effect of light quality on the morphogenesis of in vitro cultures of Azorina vidalii (Wats.) Feer
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DOI：
10.1023/a:1005988621036
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发表时间：
1997-01-01
影响因子：
3
通讯作者：
Debergh, PC
中科院分区：
文献类型：
--
作者：
da Silva, MHM;Debergh, PC
本研究探讨了提高满江红离体培养植株增殖率和质量的可能性。Feer被调查了。为此，研究了高、低蓝光/红光（分别为2.3和0.9）和红光/远红光（分别为1.1和0.6）比例对绿萍（Azorinavidalii（Wats.）对Feer进行了评价。在低红光/远红光比下培养的植株获得了最大的植株长度（87.9 ± 5.3 mm）（p小于或等于0.05）和节间长度（6.6 ± 0.5 mm）（p小于或等于0.05）。相反，当补充蓝光时，由于节间变短，植株高度降低。在高水平的红光/远红光下生长的植物中获得了每株植物的最高腋芽数量（2.4 +/-0.5），而在低水平的红光/远红光下培养的植物中获得了最低数量（0.7 +/-0.2）。在低水平的红光/远红光（1，522.5 +/- 129.5 mm（2））下培养的植物的叶面积增加，而蓝光/红光处理抑制了叶片的扩展。对于叶绿素a和总类胡萝卜素含量，在暴露于红光/远红光处理的植物和来自对照的植物之间观察到统计学差异（p小于或等于0.05）。
In the present study, the possibility to improve the multiplication rate and quality of in vitro cultured plants of Azorina vidalii (Wats.) Feer was investigated. For these purposes the influence of high and low ratios of blue/red light (2.3 and 0.9, respectively) and red/far red light (1.1 and 0.6, respectively) on the development of shoot cultures of Azorina vidalii (Wats.) Feer was evaluated. Maximum plant length (87.9 +/- 5.3 mm) (p less than or equal to 0.05) and internode length (6.6 +/- 0.5 mm) (p less than or equal to 0.05) were obtained for the plants cultured under the low ratio of red/far red light. In contrast, when blue light was supplemented, plant height was reduced, due to shorter internodes. Highest number of axillary shoots per plant (2.4 +/- 0.5) was obtained in the plants growing under a high level of red/far red light, while the lowest number (0.7 +/- 0.2) was obtained in the plants cultured under the low level of red/far red light. Leaf area was increased in plants cultured under a low level of red/far red (1,522.5 +/- 129.5 mm(2)) while blue/red light treatments inhibited leaf expansion. For chlorophyll a and total carotenoid contents, statistical differences (p less than or equal to 0.05) were observed between the plants exposed to the red/far red light treatments and the plants from the control.